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Sequence Information

Afamin (AFM) Recombinant Protein | AFM recombinant protein

Recombinant Afamin (AFM)

Gene Names
AFM; ALF; ALB2; ALBA
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Afamin (AFM); Recombinant Afamin (AFM); AFM recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 95%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200 ug/mL (varies by lot)
Applicable Applications for AFM recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Leu22~Thr210
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.8
Usage
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

Sequence Information

SDS-Page

(Figure. SDS-PAGE)

SDS-Page (Figure. SDS-PAGE)

Testing Data

(Figure. Gene Sequencing (Extract))

Testing Data (Figure. Gene Sequencing (Extract))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
173
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 23.2 kDa
Accurate: 23kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
afamin
NCBI Official Synonym Full Names
afamin
NCBI Official Symbol
AFM
NCBI Official Synonym Symbols
ALF; ALB2; ALBA
NCBI Protein Information
afamin; alpha-Alb; alpha-albumin
UniProt Protein Name
Afamin
Protein Family
UniProt Gene Name
AFM
UniProt Synonym Gene Names
ALB2; ALBA; Alpha-Alb
UniProt Entry Name
AFAM_HUMAN

NCBI Description

This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]

Uniprot Description

A-albumin: Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted, signal peptide; Secreted; Carrier

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: vitamin E binding

Biological Process: vitamin transport

Research Articles on AFM

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Product Notes

The AFM afm (Catalog #AAA2011389) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Afamin (AFM) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB) (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the AFM afm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Afamin (AFM), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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