Rabbit 5-hydroxy tryptamine (5-ht) ELISA Kit | 5-ht elisa kit

Rabbit 5-hydroxy tryptamine (5-ht) ELISA Kit

Cat. Num. AAA2600788

• Gene Names: HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
• Reactivity: Rabbit
• Synonyms: 5-hydroxy tryptamine (5-ht); Rabbit 5-hydroxy tryptamine (5-ht) ELISA Kit; 5-ht elisa kit

• Reactivity: Rabbit
• Specificity: No cross-reaction with other factors.
• Sequence Length: 422

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant 5-ht concentration.
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for 5-ht elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit 5-ht monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 53748104
• NCBI GeneID: 3350
• UniProt Accession #: Q5ZGX3; P08908
• Molecular Weight: 46,107 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 1A
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled
• NCBI Official Symbol: HTR1A
• NCBI Official Synonym Symbols: G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
• NCBI Protein Information: 5-hydroxytryptamine receptor 1A; 5-HT1a receptor; guanine nucleotide-binding regulatory protein-coupled receptor
• UniProt Protein Name: 5-hydroxytryptamine (Serotonin) receptor 1A
• UniProt Gene Name: 5HT1A
• UniProt Synonym Gene Names: HTR1A; hCG_16836
• UniProt Entry Name: Q5ZGX3_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]

Uniprot Description

5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q11.2-q13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; serotonin receptor activity

Biological Process: cell proliferation; G-protein coupled receptor protein signaling pathway; vasoconstriction; behavioral fear response; serotonin receptor, adenylate cyclase inhibiting pathway; regulation of serotonin secretion; regulation of dopamine metabolic process; positive regulation of cell proliferation; regulation of hormone secretion; serotonin metabolic process; serotonin receptor signaling pathway; regulation of behavior

Disease: Periodic Fever, Menstrual Cycle-dependent