Rat 14-3-3 protein zeta/delta ELISA Kit | Ywhaz elisa kit

Rat 14-3-3 protein zeta/delta ELISA Kit

Cat. Num. AAA760952

• Gene Names: YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
• Reactivity: Rat
• Synonyms: 14-3-3 protein zeta/delta; Rat 14-3-3 protein zeta/delta ELISA Kit; Ywhaz/Msfs1/Mitochondrial import stimulation factor S1 subunit/Protein kinase C inhibitor protein 1/KCIP-1/14-3-3 protein zeta/delta; Ywhaz elisa kit

• Reactivity: Rat
• Sequence Length: 245

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ywhaz elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Ywhaz antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Ywhaz antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Ywhaz amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Ywhaz can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 208973244
• NCBI GeneID: 7534
• NCBI Accession #: NP_001129174.1
• NCBI GenBank Nucleotide #: NM_001135702.1
• Molecular Weight: 19,333 Da
• NCBI Official Full Name: 14-3-3 protein zeta/delta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
• NCBI Protein Information: 14-3-3 protein zeta/delta
• UniProt Protein Name: 14-3-3 protein zeta/delta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAZ
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433Z_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space; focal adhesion; melanosome; mitochondrion; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein domain specific binding; protein kinase binding; transcription factor binding; ubiquitin protein ligase binding

Biological Process: apoptosis; blood coagulation; establishment of Golgi localization; gene expression; negative regulation of apoptosis; platelet activation; programmed cell death; protein targeting; regulation of mRNA stability; signal transduction; small GTPase mediated signal transduction; transcription initiation from RNA polymerase II promoter