Rabbit anti-Human, Mouse Akt Polyclonal Antibody | anti-Akt1 antibody

Akt, phosphorylated (Ser473) (Rac PKa, PKBa)

Cat. Num. AAA629476

• Gene Names: Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Akt; Polyclonal Antibody; phosphorylated (Ser473) (Rac PKa; PKBa); Anti -Akt; anti-Akt1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous human and mouse total Akt, Akt and Akt proteins when phosphorylated at Ser473.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-Akt1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.05-0.2ug/ml; Western Blot: 0.5-2.0ug/ml
• Immunogen: Synthesized phosphopeptide (KLH conjugated) derived from human Akt around the phosphorylation site of serine 473 (Q-F-S473-Y-S). Peptide is identical to the sequence surrounding S474 of human, rat and mouse Akt beta (Akt2) and S472 of human and mouse Akt gamma (Akt3).
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of cell lysates from HEK-293 (lane 1 and 3) and NIH/3T3 (lane 2 and 4) cells, using Rabbit Anti-Akt (Ser473) Polyclonal Antibody . Primary antibody: Lane 1 and 2: Rabbit IgG Control Lane 3 and 4: Rabbit Anti-Akt (Ser473) Polyclonal Antibody Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody The signal was developed with LumiSensorTM HRP Substrate Kit)

Related Product Information for anti-Akt1 antibody

Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately 10-fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt. Phosphorylation at Thr309 and Ser474 on Akt 1 and 2, and on Thr305 on Akt result in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including Bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3 specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.

Product Categories/Family for anti-Akt1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 24647360
• NCBI GeneID: 41957
• NCBI Accession #: NP_732115.1
• NCBI GenBank Nucleotide #: NM_169707.1
• UniProt Accession #: Q8INB9; Q0KI65; Q24293; Q24469; Q24470; Q7JN11; Q8T9A5; Q9VEY7
• Molecular Weight: 68,485 Da
• NCBI Official Full Name: Akt1, isoform B
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
• NCBI Protein Information: CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
• UniProt Protein Name: RAC serine/threonine-protein kinase
• Protein Family: AKT-interacting protein
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: DAkt; DRAC-PK; Dakt1; PKB
• UniProt Entry Name: AKT1_DROME

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Oncoprotein; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; phosphoinositide binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; oogenesis; positive regulation of cell size; regulation of dendrite development; chitin-based embryonic cuticle biosynthetic process; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; positive regulation of organ growth; positive regulation of cell growth; epithelial cell migration, open tracheal system; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; lipid metabolic process; response to oxidative stress; regulation of cell size; negative regulation of apoptosis