Rat Fibulin 1 (FBLN1) DIY ELISA Kit | FBLN1 diy elisa kit

Rat Fibulin 1 (FBLN1) ELISA Kit DIY Materials

Cat. Num. AAA2089283

• Gene Names: FBLN1; FBLN; FIBL1
• Reactivity: Rat
• Synonyms: Fibulin 1 (FBLN1); Rat Fibulin 1 (FBLN1) ELISA Kit DIY Materials; FBLN1 diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Fibulin 1 (FBLN1). No significant cross-reactivity or interference between Fibulin 1 (FBLN1) and analogues was observed.
• Sequence Length: 683

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for FBLN1 diy elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 18490682
• NCBI GeneID: 2192
• Molecular Weight: 74,434 Da
• NCBI Official Full Name: Fibulin 1
• NCBI Official Synonym Full Names: fibulin 1
• NCBI Official Symbol: FBLN1
• NCBI Official Synonym Symbols: FBLN; FIBL1
• NCBI Protein Information: fibulin-1
• UniProt Protein Name: Fibulin-1
• UniProt Gene Name: FBLN1
• UniProt Synonym Gene Names: FIBL-1

NCBI Description

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: extracellular matrix; extracellular region; extracellular space; fibrinogen complex; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; extracellular matrix structural constituent; fibronectin binding; identical protein binding; protein C-terminus binding; protein complex binding

Biological Process: integrin-mediated signaling pathway; negative regulation of cell adhesion; negative regulation of protein amino acid phosphorylation

Disease: Synpolydactyly 2