Testing Data
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AluI Restriction Enzyme
AluI
Purity
Molecular Biology Grade
Synonyms
AluI; AluI restriction enzyme
Host
Arthrobacter luteus
Purity/Purification
Molecular Biology Grade
Form/Format
10mM Tris-HCl (pH7.5), 50mM KCl, 0.15% Triton X-100, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Dilutent Buffer
10mM Tris-HCl (pH 7.4), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol
Methylation Effects
Dam, Dcm, CpG, EcoKl: Never overlaps-no effect.
EcoBl: may overlap-blocked
EcoBl: may overlap-blocked
Supplied With
MBS638383-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37 degree C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with AluI approximately 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.4uM. More than 95% of these can be recut.
Ligation/Recleave Assay
The ligation and recleavage assay was replaced with LO test after validating experiments showed LO tests ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with AluI.
Lambda
143
PhiX174
24
pBR322
1
pUC5
16
pUC18/19
16
pTZ19R/U
1
M13mp18/19
27
Stability During Prolonged Incubation
A minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C. Thermal Inactivation Enzyme is inactivated by incubation at 65 degree C for 20min.
Storage Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
Protocol for Digestion
Add:
Nuclease free water: 16ul
MBS638383: 2ul
DNA (0.5-1ug/ul): ul
MBS638492: 0.5-2ul
Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours.
Nuclease free water: 16ul
MBS638383: 2ul
DNA (0.5-1ug/ul): ul
MBS638492: 0.5-2ul
Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours.
Protocol for Digestion Directly after Amplification
Add:
PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA)
Nuclease free water: 18ul
MBS638383: 2ul
MBS638492: 0.5-2ul
Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours.
PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA)
Nuclease free water: 18ul
MBS638383: 2ul
MBS638492: 0.5-2ul
Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours.
Preparation and Storage
-20 degree C
Testing Data
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Testing Data
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Related Product Information for AluI restriction enzyme
5'-AGC T-3'
3'-TC^G A-5'
3'-TC^G A-5'
Product Categories/Family for AluI restriction enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
AluI
Uniprot Description
Protein family/group databasesRestriction enzymes and methylases database<br/><a href='/database/89'>More..</a>REBASEi61. AluI.
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Product Notes
The AluI (Catalog #AAA638492) is a Restriction Enzyme produced from Arthrobacter luteus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AluI, Restriction Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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