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Rat Calcineurin (CaN) DIY ELISA Kit | CaN diy elisa kit

Rat Calcineurin (CaN) ELISA Kit DIY Materials

Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
Reactivity
Rat
Synonyms
Calcineurin (CaN); Rat Calcineurin (CaN) ELISA Kit DIY Materials; PPP3CA; PPP3-CA; CCN1; CNA1; CALN; CALNA1; PP2B; PP2-B; Protein Phosphatase 3(formerly 2B); Catalytic Subunit; Alpha Isoformprotein Phosphatase 2B; Calcineurin A Alpha; CaN diy elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Calcineurin (CaN). No significant cross-reactivity or interference between Calcineurin (CaN) and analogues was observed.
Sequence Length
521
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.104ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for CaN diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,245 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
NCBI Official Synonym Full Names
protein phosphatase 3 catalytic subunit alpha
NCBI Official Symbol
PPP3CA
NCBI Official Synonym Symbols
CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
NCBI Protein Information
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Gene Name
PPP3CA
UniProt Synonym Gene Names
CALNA; CNA

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: calcineurin complex; cytoplasm; cytosol; nucleoplasm

Molecular Function: calmodulin binding; calmodulin-dependent protein phosphatase activity; drug binding; enzyme binding; protein binding; protein dimerization activity

Biological Process: calcineurin-NFAT signaling pathway; dephosphorylation; positive regulation of NFAT protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; response to calcium ion; T cell activation; Wnt receptor signaling pathway, calcium modulating pathway

Research Articles on CaN

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Product Notes

The Rat CaN ppp3ca (Catalog #AAA2089078) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089078 DIY ELISA Kit recognizes Rat CaN. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the CaN ppp3ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcineurin (CaN), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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