Rat Growth Hormone (GH) CLIA Kit | GH clia kit

Growth Hormone (GH) CLIA Kit

Cat. Num. AAA2709353

• Gene Names: Gh1; Gh; RNGHGP
• Reactivity: Rat
• Applications: Chemiluminescent Immunoassay (CLIA)
• Synonyms: Growth Hormone (GH); Growth Hormone (GH) CLIA Kit; GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone; Normal; GH clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of growth hormone. No significant cross-reactivity or interference between growth hormone and analogues was observed.

• Applicable Applications for GH clia kit: Chemiluminescent Immunoassay (CLIA)
• Application Notes: Chemiluminescent immunoassay for Antigen Detection.
• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 16-10,000pg/mL
• Sensitivity: < 5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level growth hormone were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level growth hormone were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GH clia kit

Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of growth hormone in rat serum, plasma and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to growth hormone. A competitive inhibition reaction is launched between biotin labeled growth hormone and unlabeled growth hormone (Standards or samples) with the pre-coated antibody specific to growth hormone. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of growth hormone in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the growth hormone level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 291463289
• NCBI GeneID: 24391
• NCBI Accession #: NP_001030020.2
• NCBI GenBank Nucleotide #: NM_001034848.2
• UniProt Accession #: P01244
• Molecular Weight: 24,656 Da
• NCBI Official Full Name: somatotropin
• NCBI Official Synonym Full Names: growth hormone 1
• NCBI Official Symbol: Gh1
• NCBI Official Synonym Symbols: Gh; RNGHGP
• NCBI Protein Information: somatotropin
• UniProt Protein Name: Somatotropin
• Protein Family: Envelope glycoprotein
• UniProt Gene Name: Gh1
• UniProt Synonym Gene Names: Gh
• UniProt Entry Name: SOMA_RAT

NCBI Description

ligand for growth hormone receptor; plays a role in a number of signaling pathways [RGD, Feb 2006]

Uniprot Description

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.