Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit | CLCF1 clia kit

Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit

Cat. Num. AAA2530474

• Gene Names: Clcf1; CLC; Bsf3; BSF-3; NNT-1
• Reactivity: Rat
• Synonyms: CLCF1 (Cardiotrophin Like Cytokine Factor 1); Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit; CLCF1 clia kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant CLCF1. No significant cross-reactivity or interference between CLCF1 and analogues was observed.
• Sequence Length: 225

• Assay Type: Sandwich
• Detection Range: 12.5-800pg/mL
• Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLCF1 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of CLCF1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to CLCF1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CLCF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CLCF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of CLCF1. You can calculate the concentration of CLCF1 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9910314
• NCBI GeneID: 56708
• NCBI Accession #: NP_064336.1
• NCBI GenBank Nucleotide #: NM_019952.5
• Molecular Weight: 25,261 Da
• NCBI Official Full Name: cardiotrophin-like cytokine factor 1 isoform 1
• NCBI Official Synonym Full Names: cardiotrophin-like cytokine factor 1
• NCBI Official Symbol: Clcf1
• NCBI Official Synonym Symbols: CLC; Bsf3; BSF-3; NNT-1
• NCBI Protein Information: cardiotrophin-like cytokine factor 1
• UniProt Protein Name: Cardiotrophin-like cytokine factor 1
• Protein Family: Cardiotrophin-like cytokine factor
• UniProt Gene Name: Clcf1
• UniProt Synonym Gene Names: Bsf3; Nnt1; BSF-3; NNT-1
• UniProt Entry Name: CLCF1_MOUSE

Uniprot Description

CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Secreted, signal peptide; Cytokine; Secreted

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: protein binding; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding

Biological Process: positive regulation of T-helper 2 type immune response; regulation of protein kinase B signaling cascade; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of corticotropin-releasing hormone secretion; positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; B cell differentiation; positive regulation of cell proliferation; regulation of inflammatory response; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation