Typical Testing Data/Standard Curve (for reference only)
Mouse Cardiotrophin Like Cytokine Factor 1 (CLCF1) ELISA Kit | CLCF1 elisa kit
Mouse Cardiotrophin Like Cytokine Factor 1 (CLCF1) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CLCF1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CLCF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLCF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLCF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Apoptosis; Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region; intracellular
Molecular Function: protein binding; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding
Biological Process: positive regulation of T-helper 2 type immune response; regulation of protein kinase B signaling cascade; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of corticotropin-releasing hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; B cell differentiation; positive regulation of cell proliferation; regulation of inflammatory response; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation
Research Articles on CLCF1
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Product Notes
The Mouse CLCF1 clcf1 (Catalog #AAA2023014) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023014 ELISA Kit recognizes Mouse CLCF1. It is sometimes possible for the material contained within the vial of "Cardiotrophin Like Cytokine Factor 1 (CLCF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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