Typical Testing Data/Standard Curve (for reference only)
Rat Anti-Mullerian Hormone (AMH) CLIA Kit | AMH clia kit
Rat Anti-Mullerian Hormone (AMH) CLIA Kit
Gene Names
Amh; MIS
Reactivity
Rat
Synonyms
Anti-Mullerian Hormone (AMH); Rat Anti-Mullerian Hormone (AMH) CLIA Kit; MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance; AMH clia kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH). No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
Sequence Length
553
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Biological Samples Of Humans
Assay Type
Sandwich
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMH clia kit
Intended Uses: The Ceruloplasmin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring ceruloplasmin in biological samples of humans.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CP present in samples reacts with the anti-CP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-CP is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CP in the test sample. The quantity of CP in the sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution!!Background/Introduction: Ceruloplasmin is a multifunctional protein involved in copper transport and is also an important serum antioxidant. During inflammation, traumatic injury, or myocardial infarction, the serum levels of ceruloplasmin, as an acute phase reactant rise 2-3 fold (Misra et al. 1978: Singh 1992).
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CP present in samples reacts with the anti-CP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-CP is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CP in the test sample. The quantity of CP in the sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution!!Background/Introduction: Ceruloplasmin is a multifunctional protein involved in copper transport and is also an important serum antioxidant. During inflammation, traumatic injury, or myocardial infarction, the serum levels of ceruloplasmin, as an acute phase reactant rise 2-3 fold (Misra et al. 1978: Singh 1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,889 Da
NCBI Official Full Name
muellerian-inhibiting factor
NCBI Official Synonym Full Names
anti-Mullerian hormone
NCBI Official Symbol
Amh
NCBI Official Synonym Symbols
MIS
NCBI Protein Information
muellerian-inhibiting factor
UniProt Protein Name
Muellerian-inhibiting factor
Protein Family
UniProt Gene Name
Amh
UniProt Synonym Gene Names
AMH; MIS
NCBI Description
ligand for MIS receptor II that suppresses female primary and secondary sexual development and allows male sexual differentiation to occur [RGD, Feb 2006]
Uniprot Description
This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
Research Articles on AMH
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Product Notes
The Rat AMH amh (Catalog #AAA2104774) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104774 CLIA Kit recognizes Rat AMH. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the AMH amh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Anti-Mullerian Hormone (AMH), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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