Rat TNF-a Antibody | anti-TNF-a antibody
Rat Anti-Mouse TNF-a-LE/AF
Applications
ELISA, ELISA, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Western Blot, Neutralization Assay
Synonyms
TNF-a; Rat Anti-Mouse TNF-a-LE/AF; Tumor necrosis factor-alpha; macrophage cytotoxic factor; MCF; differentiation inducing factor; DIF; cachectin; necrosin; TNFSF-2; Rat Anti-Mouse TNF-alpha-LE/AF; anti-TNF-a antibody
Host
Rat
Isotype
IgG1kappa
Clone Number
MP6-XT22
Specificity
Mouse/Chicken TNF-alpha
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for anti-TNF-a antibody
ELISA Capture (EIA), ELISA Detection (EIA), Flow Cytometry (FC/FACS), ELISpot Capture (EIA), Immunohistochemsitry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Immunoblot (IB), Neutralization, Multiplex Detection
Application Notes
ELISA-Capture - Quality tested; ELISA-Detection - Quality testedFlow Cytometry - Quality tested; ELISpot-Capture - Reported in literature; Immunohistochemsitry-Frozen Sections - Reported in literature; Immunohistochemsitry-Paraffin Sections - Reported in literature; Immunocytochemistry - Reported in literature; Immunoblotting - Reported in literature; Neutralization - Reported in literature; Multiplex-Detection - Reported in literature
Immunogen
E. coli-expressed mouse TNF-alpha
Buffer Formulation
Supplied in PBS
Isotype Control
Rat IgG1-LE/AF
Preparation and Storage
Store at 2-8 degree C
References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2. Heinzel FP, Rerko RM, Ahmed F, Hujer AM. IFN-gamma-independent production of IL-12 during murine endotoxemia. J Immunol. 1996;157:4521-8. (ELISA-Capture)
3. Bordmann G, Rudin W, Favre N. Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections. Immunology. 1998;94:35-40. (ELISA-Capture)
4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
5. Favre N, Bordmann G, Rudin W. Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J Immunol Methods. 1997;204:57-66. (ELISPOT-Capture)
6. Tripp RA, Moore D, Jones L, Sullender W, Winter J, Anderson LJ. Respiratory syncytial virus G and/or SH protein alters Th1 cytokines, natural killer cells, and neutrophils responding to pulmonary infection in BALB/c mice. J Virol. 1999;73:7099-107. (FC)
7. Asseman C, Mauze S, Leach MW, Coffman RL, Powrie F. An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. J Exp Med. 1999;190:995-1003. (FC)
8. Satoh M, Yasunami Y, Matsuoka N, Nakano M, Itoh T, Nitta T, et al. Successful islet transplantation to two recipients from a single donor by targeting proinflammatory cytokines in mice. Transplantation. 2007;83:1085-92. (FC)
9. Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
10. Vobeta EV, Škuljec J, Gudi V, Skripuletz R, Pul R, Trebst C, et al. Characterisation of microglia during de- and remyelination: can they create a repair promoting environment?. Neurobiol Dis. 2012;45:519-28. (FC)
11. Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
12. Nakamura T, Nakao T, Yoshimura N, Ashihara E. Rapamycin prolongs cardiac allograft survival in a mouse model by inducing myeloid-derived suppressor cells. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC)
13. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
14. Sunnemark D, Ulfgren A, Örn A, Harris RA. Cytokine production in hearts of Trypanosoma cruzi-infected CBA mice: Do cytokine patterns in chronic stage reflect the establishment of myocardial pathology?. Scand J Immunol. 1996;44:421-9. (IHC-FS)
15. Marinova-Mutafchieva L, Williams RO, Mason LJ, Mauri C, Feldmann M, Maini RN. Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). Clin Exp Immunol. 1997;107:507-12. (IHC-FS)
16. Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS)
17. Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
18. Jacobs M, Brown N, Allie N, Gulert R, Ryffel B. Increased resistance to mycobacterial infection in the absence of interleukin-10. Immunology. 2000;100:494-501. (IHC-FS)
19. Williams RO, Marinova-Mutafchieva L, Feldmann M, Maini RN. Evaluation of TNF-alpha and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-alpha/anti-CD4 therapy. J Immunol. 2000;165:7240-5. (IHC-FS)
20. Sanders EJ, Prasad S, Hu N, Wride MA. Cell death in the gastrulating chick embryo: potential roles for tumor necrosis factor-alpha (TNF-alpha). Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity)
21. Wride MA, Sanders EJ. Expression of tumor necrosis factor-alpha (TNFalpha)-cross-reactive proteins during early chick embryo development. Dev Dyn. 1993;198:225-39. (ICC, WB, Chicken Reactivity)
22. Scanga CA, Mohan VP, Joseph H, Yu K, Chan J, Flynn JL. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun. 1999;67:4531-8. (Neut)
23. Via CS, Shustov A, Rus V, Lang T, Nguyen P, Finkelman FD. In vivo neutralization of TNF-alpha promotes humoral autoimmunity by preventing the induction of CTL. J Immunol. 2001;167:6821-6. (Neut)
24. Zhou R, Wei H, Sun R, Tian Z. Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007;178:4548-56. (Neut)
25. Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)
2. Heinzel FP, Rerko RM, Ahmed F, Hujer AM. IFN-gamma-independent production of IL-12 during murine endotoxemia. J Immunol. 1996;157:4521-8. (ELISA-Capture)
3. Bordmann G, Rudin W, Favre N. Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections. Immunology. 1998;94:35-40. (ELISA-Capture)
4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
5. Favre N, Bordmann G, Rudin W. Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J Immunol Methods. 1997;204:57-66. (ELISPOT-Capture)
6. Tripp RA, Moore D, Jones L, Sullender W, Winter J, Anderson LJ. Respiratory syncytial virus G and/or SH protein alters Th1 cytokines, natural killer cells, and neutrophils responding to pulmonary infection in BALB/c mice. J Virol. 1999;73:7099-107. (FC)
7. Asseman C, Mauze S, Leach MW, Coffman RL, Powrie F. An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. J Exp Med. 1999;190:995-1003. (FC)
8. Satoh M, Yasunami Y, Matsuoka N, Nakano M, Itoh T, Nitta T, et al. Successful islet transplantation to two recipients from a single donor by targeting proinflammatory cytokines in mice. Transplantation. 2007;83:1085-92. (FC)
9. Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
10. Vobeta EV, Škuljec J, Gudi V, Skripuletz R, Pul R, Trebst C, et al. Characterisation of microglia during de- and remyelination: can they create a repair promoting environment?. Neurobiol Dis. 2012;45:519-28. (FC)
11. Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
12. Nakamura T, Nakao T, Yoshimura N, Ashihara E. Rapamycin prolongs cardiac allograft survival in a mouse model by inducing myeloid-derived suppressor cells. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC)
13. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
14. Sunnemark D, Ulfgren A, Örn A, Harris RA. Cytokine production in hearts of Trypanosoma cruzi-infected CBA mice: Do cytokine patterns in chronic stage reflect the establishment of myocardial pathology?. Scand J Immunol. 1996;44:421-9. (IHC-FS)
15. Marinova-Mutafchieva L, Williams RO, Mason LJ, Mauri C, Feldmann M, Maini RN. Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). Clin Exp Immunol. 1997;107:507-12. (IHC-FS)
16. Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS)
17. Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
18. Jacobs M, Brown N, Allie N, Gulert R, Ryffel B. Increased resistance to mycobacterial infection in the absence of interleukin-10. Immunology. 2000;100:494-501. (IHC-FS)
19. Williams RO, Marinova-Mutafchieva L, Feldmann M, Maini RN. Evaluation of TNF-alpha and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-alpha/anti-CD4 therapy. J Immunol. 2000;165:7240-5. (IHC-FS)
20. Sanders EJ, Prasad S, Hu N, Wride MA. Cell death in the gastrulating chick embryo: potential roles for tumor necrosis factor-alpha (TNF-alpha). Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity)
21. Wride MA, Sanders EJ. Expression of tumor necrosis factor-alpha (TNFalpha)-cross-reactive proteins during early chick embryo development. Dev Dyn. 1993;198:225-39. (ICC, WB, Chicken Reactivity)
22. Scanga CA, Mohan VP, Joseph H, Yu K, Chan J, Flynn JL. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun. 1999;67:4531-8. (Neut)
23. Via CS, Shustov A, Rus V, Lang T, Nguyen P, Finkelman FD. In vivo neutralization of TNF-alpha promotes humoral autoimmunity by preventing the induction of CTL. J Immunol. 2001;167:6821-6. (Neut)
24. Zhou R, Wei H, Sun R, Tian Z. Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007;178:4548-56. (Neut)
25. Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)
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Product Notes
The TNF-a (Catalog #AAA670600) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNF-a can be used in a range of immunoassay formats including, but not limited to, ELISA Capture (EIA), ELISA Detection (EIA), Flow Cytometry (FC/FACS), ELISpot Capture (EIA), Immunohistochemsitry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Immunoblot (IB), Neutralization, Multiplex Detection. ELISA-Capture - Quality tested; ELISA-Detection - Quality testedFlow Cytometry - Quality tested; ELISpot-Capture - Reported in literature; Immunohistochemsitry-Frozen Sections - Reported in literature; Immunohistochemsitry-Paraffin Sections - Reported in literature; Immunocytochemistry - Reported in literature; Immunoblotting - Reported in literature; Neutralization - Reported in literature; Multiplex-Detection - Reported in literature. Researchers should empirically determine the suitability of the TNF-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF-a, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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