Testing Data
Mouse IFN-g Antibody | anti-IFN-g antibody
Mouse Anti-Human IFN-g-LE/AF
Gene Names
IFNG; IFN-g; IFN-gamma
Applications
ELISA, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Neutralization Assay
Synonyms
IFN-g; Mouse Anti-Human IFN-g-LE/AF; Interferon-gamma; gIFN; IFNG; immune interferon; IIF; type II interferon; T interferon; T cell interferon; mitogen induced interferon; pH2-labile interferon; macrophage-activating factor; MAF; Mouse Anti-Human IFN-gamma-LE/AF; anti-IFN-g antibody
Host
Mouse
Isotype
IgG1kappa
Clone Number
B27
Specificity
Human/Rhesus/Cynomolgus/Chimpanzee/Pigtail Macaque/African Green Monkey/Sooty Mangabey IFN-gamma
Concentration
0.5 mg/mL (varies by lot)
Sequence Length
166
Applicable Applications for anti-IFN-g antibody
ELISA Detection (EIA), Flow Cytometry (FC/FACS), ELISpot Capture (EIA), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunoprecipitation (IP), Neutralization, Multiplex Capture
Application Notes
ELISA-Detection - Quality tested; Flow Cytometry - Quality tested ; ELISpot-Capture - Reported in literature; Immunohistochemistry-Frozen Sections - Reported in literature; Immunocytochemistry - Reported in literature; Immunoprecipitation - Reported in literature; Neutralization - Reported in literature; Multiplex-Capture - Reported in literature;
Immunogen
E. coli-expressed human IFN-gamma
Buffer Formulation
Supplied in PBS
Isotype Control
Mouse IgG1-LE/AF
Preparation and Storage
Store at 2-8 degree C
Testing Data
Testing Data
References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
3. Lagier B, Lebel B, Bousquet J, Péne J. Different modulation by histamine of IL-4 and interferon-gamma (IFN-gamma) release according to the phenotype of human Th0, Th1 and Th2 clones. Clin Exp Immunol. 1997;108:545-51. (ELISA-Detection)
4. Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression. Am J Pathol. 2004;164:409-17. (ELISA-Detection)
5. Barouch DH, Kunstman J, Kuroda MJ, Schmitz JE, Santra S, Peyerl FW, et al. Eventual AIDS vaccine failure in a rhesus monkey by viral escape from cytotoxic T lymphocytes. Nature. 2002;415:335-9. (ELISPOT-Capture, Rhesus Reactivity)
6. Malkevitch N, Rohne D, Pinczewski J, Aldrich K, Kalyanaraman VS, Letvin NL, et al. Evaluation of combination DNA/replication-competent Ad-SIV recombinant immunization regimens in rhesus macaques. AIDS Res Hum Retroviruses. 2004;20:235-44. (ELISPOT-Capture, Rhesus Reactivity)
7. Shen L, Shen Y, Huang D, Qiu L, Sehgal P, Du GZ, et al. Development of Vgamma2Vdelta2+ T cell responses during active mycobacterial coinfection of simian immunodeficiency virus-infected macaques requires control of viral infection and immune competence of CD4+ T cells. J Infect Dis. 2004;190:1438-47. (ELISPOT-Capture, Rhesus & Pigtail Macaque Reactivity)
8. Reimann KA, Parker RA, Seaman MS, Beaudry K, Beddall M, Peterson L, et al. Pathogenicity of simian-human immunodeficiency virus SHIV-89.6P and SIVmac is attenuated in cynomolgus macaques and associated with early T-lymphocyte responses. J Virol. 2005;79:8878-85. (ELISPOT-Capture, Cynomolgus & Rhesus Reactivity)
9. Letvin NL, Rao SS, Dang V, Buzby AP, Korioth-Schmitz B, Dombagoda D, et al. No evidence for consistent virus-specific immunity in simian immunodeficiency virus-exposed, uninfected rhesus monkeys. J Virol. 2007;81:12368-74. (ELISPOT-Capture, FC, Rhesus Reactivity)
10. Zahn RC, Rett MD, Korioth-Schmitz B, Sun Y, Buzby AP, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8+ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88. (ELISPOT-Capture, FC, African Green Monkey & Rhesus Reactivity)
11. Favre C, Wijdenes J, Cabrillat H, Djossou O, Banchereau J, de Vries JE. Epitope mapping of recombinant human gamma interferon using monoclonal antibodies. Mol Immunol. 1989;26:17-25. (ELISA-Detection, IP)
12. Sumpter B, Dunham R, Gordon S, Engram J, Hennessy M, Kinter A, et al. Correlates of preserved CD4+ T cell homeostasis during natural, nonpathogenic simian immunodeficiency virus infection of sooty mangabeys: implications for AIDS pathogenesis. J Immunol. 2007;178:1680-91. (FC, Sooty Mangabey Reactivity)
13. De Rose R, Batten CJ, Smith MZ, Fernandez CS, Peut V, Thomson S, et al. Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques. J Virol. 2007;81:292-300. (FC, Pigtail Macaque Reactivity)
14. Liu J, Ewald BA, Lynch DM, Denholtz M, Abbink P, Lemckert AA, et al. Magnitude and phenotype of cellular immune responses elicited by recombinant adenovirus vectors and heterologous prime-boost regimens in rhesus monkeys. J Virol. 2008;82:4844-52. (FC, Rhesus Reactivity)
15. Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37. (FC, Cynomolgus Reactivity)
16. Wilks AB, Perry JR, Ehlinger EP, Zahn RC, White R, Gauduin M, et al. High cell-free virus load and robust autologous humoral immune responses in breast milk of simian immunodeficiency virus-infected african green monkeys. J Virol. 2011;85:9517-26. (FC, African Green Monkey Reactivity)
17. Reeves RK, Evans TI, Fultz PN, Johnson RP. Potential confusion of contaminating CD16+ myeloid DCs with anergic CD16+ NK cells in chimpanzees. Eur J Immunol. 2011;41:1070-4. (FC, Chimpanzee Reactivity)
18. Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, et al. CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques. AIDS Res Hum Retroviruses. 2012;28:1693-702. (FC, Cynomolgus Reactivity)
19. Stary G, Klein I, Brüggen M, Kohlhofer S, Brunner PM, Spazierer D, et al. Host defense mechanisms in secondary syphilitic lesions: a role for IFN-gamma-/IL-17-producing CD8+ T cells?. Am J Pathol. 2010;177:2421-32. (IHC-FS)
20. Chemin K, Bohineust A, Dogniaux S, Tourret M, Guégan S, Miro F, et al. Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol. 2012;189:2159-68. (ICC)
21. Gangur V, Simons FE, Hayglass KT. Human IP-10 selectively promotes dominance of polyclonally activated and environmental antigen-driven IFN-gamma over IL-4 responses. FASEB J. 1998;12:705-13. (Neut)
22. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)
2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
3. Lagier B, Lebel B, Bousquet J, Péne J. Different modulation by histamine of IL-4 and interferon-gamma (IFN-gamma) release according to the phenotype of human Th0, Th1 and Th2 clones. Clin Exp Immunol. 1997;108:545-51. (ELISA-Detection)
4. Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression. Am J Pathol. 2004;164:409-17. (ELISA-Detection)
5. Barouch DH, Kunstman J, Kuroda MJ, Schmitz JE, Santra S, Peyerl FW, et al. Eventual AIDS vaccine failure in a rhesus monkey by viral escape from cytotoxic T lymphocytes. Nature. 2002;415:335-9. (ELISPOT-Capture, Rhesus Reactivity)
6. Malkevitch N, Rohne D, Pinczewski J, Aldrich K, Kalyanaraman VS, Letvin NL, et al. Evaluation of combination DNA/replication-competent Ad-SIV recombinant immunization regimens in rhesus macaques. AIDS Res Hum Retroviruses. 2004;20:235-44. (ELISPOT-Capture, Rhesus Reactivity)
7. Shen L, Shen Y, Huang D, Qiu L, Sehgal P, Du GZ, et al. Development of Vgamma2Vdelta2+ T cell responses during active mycobacterial coinfection of simian immunodeficiency virus-infected macaques requires control of viral infection and immune competence of CD4+ T cells. J Infect Dis. 2004;190:1438-47. (ELISPOT-Capture, Rhesus & Pigtail Macaque Reactivity)
8. Reimann KA, Parker RA, Seaman MS, Beaudry K, Beddall M, Peterson L, et al. Pathogenicity of simian-human immunodeficiency virus SHIV-89.6P and SIVmac is attenuated in cynomolgus macaques and associated with early T-lymphocyte responses. J Virol. 2005;79:8878-85. (ELISPOT-Capture, Cynomolgus & Rhesus Reactivity)
9. Letvin NL, Rao SS, Dang V, Buzby AP, Korioth-Schmitz B, Dombagoda D, et al. No evidence for consistent virus-specific immunity in simian immunodeficiency virus-exposed, uninfected rhesus monkeys. J Virol. 2007;81:12368-74. (ELISPOT-Capture, FC, Rhesus Reactivity)
10. Zahn RC, Rett MD, Korioth-Schmitz B, Sun Y, Buzby AP, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8+ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88. (ELISPOT-Capture, FC, African Green Monkey & Rhesus Reactivity)
11. Favre C, Wijdenes J, Cabrillat H, Djossou O, Banchereau J, de Vries JE. Epitope mapping of recombinant human gamma interferon using monoclonal antibodies. Mol Immunol. 1989;26:17-25. (ELISA-Detection, IP)
12. Sumpter B, Dunham R, Gordon S, Engram J, Hennessy M, Kinter A, et al. Correlates of preserved CD4+ T cell homeostasis during natural, nonpathogenic simian immunodeficiency virus infection of sooty mangabeys: implications for AIDS pathogenesis. J Immunol. 2007;178:1680-91. (FC, Sooty Mangabey Reactivity)
13. De Rose R, Batten CJ, Smith MZ, Fernandez CS, Peut V, Thomson S, et al. Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques. J Virol. 2007;81:292-300. (FC, Pigtail Macaque Reactivity)
14. Liu J, Ewald BA, Lynch DM, Denholtz M, Abbink P, Lemckert AA, et al. Magnitude and phenotype of cellular immune responses elicited by recombinant adenovirus vectors and heterologous prime-boost regimens in rhesus monkeys. J Virol. 2008;82:4844-52. (FC, Rhesus Reactivity)
15. Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37. (FC, Cynomolgus Reactivity)
16. Wilks AB, Perry JR, Ehlinger EP, Zahn RC, White R, Gauduin M, et al. High cell-free virus load and robust autologous humoral immune responses in breast milk of simian immunodeficiency virus-infected african green monkeys. J Virol. 2011;85:9517-26. (FC, African Green Monkey Reactivity)
17. Reeves RK, Evans TI, Fultz PN, Johnson RP. Potential confusion of contaminating CD16+ myeloid DCs with anergic CD16+ NK cells in chimpanzees. Eur J Immunol. 2011;41:1070-4. (FC, Chimpanzee Reactivity)
18. Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, et al. CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques. AIDS Res Hum Retroviruses. 2012;28:1693-702. (FC, Cynomolgus Reactivity)
19. Stary G, Klein I, Brüggen M, Kohlhofer S, Brunner PM, Spazierer D, et al. Host defense mechanisms in secondary syphilitic lesions: a role for IFN-gamma-/IL-17-producing CD8+ T cells?. Am J Pathol. 2010;177:2421-32. (IHC-FS)
20. Chemin K, Bohineust A, Dogniaux S, Tourret M, Guégan S, Miro F, et al. Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol. 2012;189:2159-68. (ICC)
21. Gangur V, Simons FE, Hayglass KT. Human IP-10 selectively promotes dominance of polyclonally activated and environmental antigen-driven IFN-gamma over IL-4 responses. FASEB J. 1998;12:705-13. (Neut)
22. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,434 Da
NCBI Official Full Name
interferon gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFN-g; IFN-gamma
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_BUBBU
Uniprot Description
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons ().
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Product Notes
The IFN-g ifng (Catalog #AAA670601) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IFN-g can be used in a range of immunoassay formats including, but not limited to, ELISA Detection (EIA), Flow Cytometry (FC/FACS), ELISpot Capture (EIA), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunoprecipitation (IP), Neutralization, Multiplex Capture. ELISA-Detection - Quality tested; Flow Cytometry - Quality tested ; ELISpot-Capture - Reported in literature; Immunohistochemistry-Frozen Sections - Reported in literature; Immunocytochemistry - Reported in literature; Immunoprecipitation - Reported in literature; Neutralization - Reported in literature; Multiplex-Capture - Reported in literature;. Researchers should empirically determine the suitability of the IFN-g ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-g, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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