Retinoic Acid Receptor Alpha (RARa) Recombinant Protein | RARa recombinant protein

Recombinant Retinoic Acid Receptor Alpha (RARa)

Cat. Num. AAA2011319

• Gene Names: Rara; RAR; Nr1b1; RARalpha1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Retinoic Acid Receptor Alpha (RARa); Recombinant Retinoic Acid Receptor Alpha (RARa); RARa recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VEFA KQLPGFTTLT IADQITLLKA ACLDILILRI CTRYTPEQDT MTFSDGLTLN RTQMHNAGFG PLTDLVFAFA NQLLPLEMDD AETGLLSAIC LICGDRQDLE QPDKVDMLQE PLLEALKVYV RKRRPSRPHM FPKMLMKITD LRSISAKGAE RVITLKMEIP GSMPPLIQEM LENSEGLDTL SGQSGGGTRD GGGLAPPPGS CSPSLSPSSH RSSPATQSP
• Sequence Length: 459

• Applicable Applications for RARa recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val237~Pro459 (Accession # P11416) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus. Cytoplasm
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 293335963
• NCBI GeneID: 19401
• NCBI Accession #: NP_001169999.1
• NCBI GenBank Nucleotide #: NM_001176528.1
• UniProt Accession #: P11416; P22603
• Molecular Weight: 28.0kDa
• NCBI Official Full Name: retinoic acid receptor alpha isoform 2
• NCBI Official Synonym Full Names: retinoic acid receptor, alpha
• NCBI Official Symbol: Rara
• NCBI Official Synonym Symbols: RAR; Nr1b1; RARalpha1
• NCBI Protein Information: retinoic acid receptor alpha; RAR-alpha; RAR alpha 1; nuclear receptor subfamily 1 group B member 1
• UniProt Protein Name: Retinoic acid receptor alpha
• Protein Family: Retinoic acid receptor
• UniProt Gene Name: Rara
• UniProt Synonym Gene Names: Nr1b1; RAR-alpha
• UniProt Entry Name: RARA_MOUSE

Uniprot Description

RARA: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARA is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Its phosphorylation is crucial for transcriptional activity. Aberrations involving RARA may be a cause of acute promyelocytic leukemia. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor; Oncoprotein; Nuclear receptor

Cellular Component: neuron projection; cell soma; perinuclear region of cytoplasm; nuclear chromatin; dendrite; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: protein domain specific binding; protein kinase B binding; ligand-dependent nuclear receptor activity; retinoic acid binding; zinc ion binding; chromatin DNA binding; metal ion binding; histone deacetylase binding; transcription coactivator activity; translation repressor activity, nucleic acid binding; phosphoinositide 3-kinase regulator activity; drug binding; alpha-actinin binding; transcription factor binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein kinase A binding; steroid hormone receptor activity; mRNA 5'-UTR binding; retinoic acid receptor activity; transcription corepressor activity; transcription factor activity; receptor binding

Biological Process: retinoic acid receptor signaling pathway; limb development; transcription from RNA polymerase II promoter; negative regulation of translational initiation; estrogen receptor signaling pathway; regulation of myelination; glandular epithelial cell development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell differentiation; regulation of synaptic plasticity; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; response to estradiol stimulus; negative regulation of granulocyte differentiation; germ cell development; regulation of apoptosis; positive regulation of interleukin-4 production; negative regulation of cell proliferation; Sertoli cell fate commitment; positive regulation of T-helper 2 cell differentiation; regulation of transcription, DNA-dependent; ureteric bud development; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; response to retinoic acid; transcription, DNA-dependent; multicellular organism growth; male gonad development; positive regulation of cell cycle; negative regulation of tumor necrosis factor production; positive regulation of binding; embryonic camera-type eye development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of cell differentiation; response to ethanol; regulation of granulocyte differentiation; negative regulation of translation; neural tube closure; spermatogenesis; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; apoptotic cell clearance; negative regulation of apoptosis