V-ral Simian Leukemia Viral Oncogene Homolog A Recombinant Protein | RALA recombinant protein

Recombinant Human V-ral Simian Leukemia Viral Oncogene Homolog A

Cat. Num. AAA144169

• Gene Names: RALA; RAL
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: V-ral Simian Leukemia Viral Oncogene Homolog A; Recombinant Human V-ral Simian Leukemia Viral Oncogene Homolog A; RALA Human; V-ral Simian Leukemia Viral Oncogene Homolog A Human Recombinant; Ras-related protein Ral-A; RALA; RAL; MGC48949; RALA recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The RALA solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT, 100mM NaCl and 0.1mM PMSF.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA EQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC
• Sequence Length: 206

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for RALA recombinant protein

Description: RALA Human Recombinant fused with a 24 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 227 amino acids (1-203 a.a.) and having a molecular mass of 25.8kDa. The RALA is purified by proprietary chromatographic techniques.

Introduction: Ras-related protein Ral-A (RALA) is a member of the small GTPase superfamily, Ras family of proteins. RALA mediates a distinct downstream signaling pathway from Ras which facilitates cellular transformation. Furthermore, RALA is thought to be involved in various signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis.

Product Categories/Family for RALA recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; V-ral Simian Leukemia Viral Oncogene

NCBI and Uniprot Product Information

• NCBI GI #: 33946329
• NCBI GeneID: 5898
• NCBI Accession #: NP_005393.2
• NCBI GenBank Nucleotide #: NM_005402.3
• UniProt Accession #: P11233; A4D1W3
• Molecular Weight: 23,567 Da
• NCBI Official Full Name: ras-related protein Ral-A
• NCBI Official Synonym Full Names: v-ral simian leukemia viral oncogene homolog A (ras related)
• NCBI Official Symbol: RALA
• NCBI Official Synonym Symbols: RAL
• NCBI Protein Information: ras-related protein Ral-A; RAS-like protein A; Ras family small GTP binding protein RALA; ras related GTP binding protein A
• UniProt Protein Name: Ras-related protein Ral-A
• Protein Family: Ras-related protein
• UniProt Gene Name: RALA
• UniProt Synonym Gene Names: RAL
• UniProt Entry Name: RALA_HUMAN

NCBI Description

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: cytoplasmic vesicle membrane; cell surface; focal adhesion; endocytic vesicle; plasma membrane; midbody; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; myosin binding; GTP binding; Edg-2 lysophosphatidic acid receptor binding; ubiquitin protein ligase binding; ATPase binding

Biological Process: positive regulation of filopodium formation; regulation of exocytosis; exocytosis; nerve growth factor receptor signaling pathway; lipid raft localization; viral reproduction; metabolic process; Ras protein signal transduction; actin cytoskeleton reorganization; cytokinesis; signal transduction; chemotaxis