Retinoic Acid Early Transcript 1G Recombinant Protein | RAET1G recombinant protein

Recombinant Human Retinoic Acid Early Transcript 1G

Cat. Num. AAA146578

• Gene Names: RAET1G; ULBP5
• Purity: Purity as determined by densitometric image analysis is greater than 95%.
• Synonyms: Retinoic Acid Early Transcript 1G; Recombinant Human Retinoic Acid Early Transcript 1G; RAET1G Human; Retinoic Acid Early Transcript 1G Human Recombinant; Retinoic acid early transcript 1G protein; RAET1G; ULBP5; RAET1G recombinant protein

• Host: E Coli
• Purity/Purification: Purity as determined by densitometric image analysis is greater than 95%.
• Form/Format: RAET1G filtered (0.4 um) and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl, pH 7.5.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: MKHHHHHHASGLADPHSLCY DITVIPKFRP GPRWCAVQGQ VDEKTFLHYD CGSKTVTPVS PLGKKLNVTT AWKAQNPVLR EVVDILTEQL LDIQLENYIP KEPLTLQARM SCEQKAEGHG SGSWQLSFDG QIFLLFDSEN RMWTTVHPGA RKMKEKWEND KDMTMSFHYI SMGDCTGWLE DFLMGMDSTL EPSAGGTV.
• Sequence Length: 334

• Solubility: It is recommended to add 200 ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. RAET1G is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for RAET1G recombinant protein

Description: RAET1G Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (corresponding to Isoform 2, Uniprot accession #Q6H3X3-2) containing 198 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 22.43kDa (calculated).

Introduction: Retinoic Acid Early Transcript 1G (RAET1G/ULBP5) is a member of the MHC class I family. RAET1G functions as a ligand for the NKG2D receptor on the surface of NK cells. NKG2D stimulation influences anti-tumor and anti-viral immune responses of NK cells. ULBP ligands are generated by virally infected cells and tumors. RAET1G is expressed in 2 variants- a membrane and a soluble isoform. RAET1G is highly expressed in the colon and in several tumor cell lines.

Product Categories/Family for RAET1G recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 130484306
• NCBI GeneID: 353091
• NCBI Accession #: NP_001001788.2
• NCBI GenBank Nucleotide #: NM_001001788.3
• UniProt Accession #: Q6H3X3; Q6H3X2
• Molecular Weight: 23,884 Da
• NCBI Official Full Name: retinoic acid early transcript 1G protein preproprotein
• NCBI Official Synonym Full Names: retinoic acid early transcript 1G
• NCBI Official Symbol: RAET1G
• NCBI Official Synonym Symbols: ULBP5
• NCBI Protein Information: retinoic acid early transcript 1G protein; UL-16 binding protein 5
• UniProt Protein Name: Retinoic acid early transcript 1G protein
• Protein Family: Retinoic acid early transcript 1G protein
• UniProt Gene Name: RAET1G
• UniProt Synonym Gene Names: ULBP5
• UniProt Entry Name: RET1G_HUMAN

NCBI Description

Members of the RAET1 family, such as RAET1G, are major histocompatibility complex (MHC) class I-related genes located within a 180-kb cluster on chromosome 6q24.2-q25.3. RAET1 proteins contain MHC class I-like alpha-1 and alpha-2 domains. RAET1E (MIM 609243) and RAET1G differ from the other RAET1 proteins (e.g., RAET1I, or ULBP1; MIM 605697) in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAET1G: Acts as a ligand for the NKG2D receptor and mediates NK cell cytotoxicity via the receptor. Belongs to the MHC class I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q24.1-q25.1

Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity