Western Blot (WB)
(Western Blot detection against Immunogen (36.56kD).)
Mouse anti-Human CHST12 Monoclonal Antibody | anti-CHST12 antibody
CHST12 (Carbohydrate Sulfotransferase 12, Chondroitin 4-O-sulfotransferase 2, Chondroitin 4-sulfotransferase 2, C4ST-2, C4ST2, Sulfotransferase Hlo, UNQ500/PRO1017)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot detection against Immunogen (36.56kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.56kD).)
Western Blot (WB)
(Western Blot analysis of CHST12 expression in transfected 293T cell line by CHST12 monoclonal antibody.|Lane 1: CHST12 transfected lysate (48.4kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CHST12 expression in transfected 293T cell line by CHST12 monoclonal antibody.|Lane 1: CHST12 transfected lysate (48.4kD).|Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged CHST12 is 0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged CHST12 is 0.03ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5' UTRs have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
Function: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Catalytic activity: 3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate. Ref.1
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein
By similarity.
Tissue specificity: Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung. Ref.1
Sequence similarities: Belongs to the sulfotransferase 2 family.
Research Articles on CHST12
Similar Products
Product Notes
The CHST12 chst12 (Catalog #AAA648912) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CHST12 (Carbohydrate Sulfotransferase 12, Chondroitin 4-O-sulfotransferase 2, Chondroitin 4-sulfotransferase 2, C4ST-2, C4ST2, Sulfotransferase Hlo, UNQ500/PRO1017) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHST12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the CHST12 chst12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDRELTADSD VDEFLDKFLS AGVKQSDLPR KETEQPPAPG SMEESVRGYD WSPRDARRSP DQGRQQAERR SVLRGFCANS SLAFPTKERA FDDIP. It is sometimes possible for the material contained within the vial of "CHST12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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