Western Blot (WB)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Numb Homolog (NUMB) Polyclonal Antibody | anti-NUMB antibody
Polyclonal Antibody to Numb Homolog (NUMB)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAKPA HTALAPVAMP VRETNPWAHA PDAANKEIAA TCSGTEWGQS SGAASPGLFQ AGHRRTPSEA
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: NUMB knockout A431 cell lysate; Predicted MW: 71kd Observed MW: 71kd Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: NUMB knockout A431 cell lysate; Predicted MW: 71kd Observed MW: 71kd Primary Ab: 2ug/ml Rabbit Anti-Human NUMB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Uniprot Description
NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 14q24.2-q24.3
Cellular Component: apical part of cell; basolateral plasma membrane; clathrin-coated vesicle; early endosome; extrinsic component of plasma membrane; focal adhesion; nucleus; plasma membrane
Molecular Function: alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding
Biological Process: axonogenesis; lateral ventricle development; negative regulation of Notch signaling pathway; neuroblast division in subventricular zone; positive regulation of cell migration; positive regulation of neurogenesis; positive regulation of polarized epithelial cell differentiation
Research Articles on NUMB
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Product Notes
The NUMB numb (Catalog #AAA2014856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Numb Homolog (NUMB) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Numb Homolog (NUMB) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NUMB numb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAKPA HTALAPVAMP VRETNPWAHA PDAANKEIAA TCSGTEWGQS SGAASPGLFQ AGHRRTPSEA. It is sometimes possible for the material contained within the vial of "Numb Homolog (NUMB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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