DNA repair protein RAD51 homolog 3 (RAD51C) Recombinant Protein | RAD51C recombinant protein

Recombinant Cricetulus griseus DNA repair protein RAD51 homolog 3 (RAD51C)

Cat. Num. AAA1281845

• Gene Names: Rad51c; R51H3; rad51L2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA repair protein RAD51 homolog 3 (RAD51C); Recombinant Cricetulus griseus DNA repair protein RAD51 homolog 3 (RAD51C); RAD51C recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-366, full length protein
• Sequence: MQRELVSFPLSPTVRVKLVAAGFQTAEDVLGVKPSELSKEVGISKEEALETLQIVRRESLTDKPRCAGASVAGKKYTALELLEQEHTQGFIITFCSALDNILGGGIPLMKTTEVCGVPGVGKTQLCMQLAVDVQIPECFGGVAGEAVFIDTEGSFMVDRVVTLANACIQHLHLIAGTHKDEEHQKALEGFTLENILSHIYYFRCHDYTELLAQVYLLPDFLSNHSKVQLVIIDGIALPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAVILTNQMTTKIDKNQALLVPALGESWGHAATIRLIFHWEQKQRFATLYKSPSQKESTIPFQITPQGFRDAAVTASSSQTEGSSNLRKRSREPEEGC
• Sequence Length: 366

• Species: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 346644687
• NCBI GeneID: 100689078
• NCBI Accession #: NP_001231005.1
• NCBI GenBank Nucleotide #: NM_001244076.1
• UniProt Accession #: Q8R2J9; Q8VDE9
• Molecular Weight: 35,464 Da
• NCBI Official Full Name: DNA repair protein RAD51 homolog 3
• NCBI Official Symbol: Rad51c
• NCBI Official Synonym Symbols: R51H3; rad51L2
• NCBI Protein Information: DNA repair protein RAD51 homolog 3
• UniProt Protein Name: DNA repair protein RAD51 homolog 3
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51C
• UniProt Synonym Gene Names: RAD51L2; R51H3

Uniprot Description

Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Part of the RAD21 paralog protein complexes BCDX2 and CX3 which act at different stages of the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 seems to act downstream of BRCA2 recruitment and upstream of RAD51 recruitment; CX3 seems to act downstream of RAD51 recruitment; both complexes bind predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. Involved in RAD51 foci formation in response to DNA damage suggesting an involvement in early stages of HR probably in the invasion step. Has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Participates in branch migration and HJ resolution and thus is important for processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex. Part of a PALB2-scaffolded HR complex containing BRCA2 and which is thought to play a role in DNA repair by HR. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.