RAC2 recombinant protein

RAC2 recombinant protein

Cat. Num. AAA515107

• Gene Names: RAC2; Gx; EN-7; HSPC022; p21-Rac2
• Applications: Western Blot
• Synonyms: RAC2; RAC2 recombinant protein; Gx; EN-7; HSPC022

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 1538

• Applicable Applications for RAC2 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant full-length human RAC2 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for RAC2 recombinant protein

Recombinant full-length human RAC2 was expressed in E. coli cells using an N-terminal His tag

Scientific Background: RAC2 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins and is involved in a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization and the activation of protein kinases. Knockdown of the gene for both RAC1 and RAC2 completely blocks B-cell development indicating that both GTPases are required to transduce B-cell receptor (BCR) signals leading to proliferation, survival, and the upregulation of BAFF receptor (1). Furthermore, RAC2 is required for the transfer of electrons from NADPH to cytochrome b-associated FAD, then to cytochrome b heme, and finally to oxygen (2).

Product Categories/Family for RAC2 recombinant protein

G-Proteins; Signaling Proteins - G-Proteins

References

1.Walmsley, M. J. et al: Critical roles for Rac1 and Rac2 GTPases in B cell development and signaling. Science 302: 459-462, 2003.
2.Diebold, B. A. et al: Molecular basis for Rac2 regulation of phogocyte NADPH oxidase. Nature Immun. 2: 211-215, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 520262262
• NCBI GeneID: 5880
• NCBI Accession #: NP_002863.1
• NCBI GenBank Nucleotide #: NM_002872
• UniProt Accession #: P15153; Q9UDJ4; AAB22207
• Molecular Weight: ~ 22 kDa
• NCBI Official Full Name: Homo sapiens ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) (RAC2), mRNA
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• NCBI Official Symbol: RAC2
• NCBI Official Synonym Symbols: Gx; EN-7; HSPC022; p21-Rac2
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 2; small G protein; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2)
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 2
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC2
• UniProt Entry Name: RAC2_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Ref.8

Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.

Subunit structure: Interacts with DOCK2, which may activate it. Interacts with S100A8 and calprotectin (S100A8/9). Ref.12 Ref.13

Subcellular location: Cytoplasm. Note: Membrane-associated when activated.

Tissue specificity: Hematopoietic specific.

Involvement in disease: Neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]: An immunodeficiency syndrome due to defective neutrophils. Affected individuals present with leukocytosis, neutrophilia, severe recurrent bacterial infections and poor wound healing.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17

Sequence similarities: Belongs to the small GTPase superfamily. Rho family.