RAC2 recombinant protein

Recombinant Human RAC2 Protein (His tag)

Cat. Num. AAA2545572

• Gene Names: RAC2; Gx; EN-7; HSPC022; p21-Rac2
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: RAC2; Recombinant Human RAC2 Protein (His tag); EN-7; Gx; HSPC022; RAC2 recombinant protein

• Host: E Coli
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH 7.5
• Sequence Length: 192

• Application Notes: The recombinant human RAC2 comprises 200 amino acids and has a predicted molecular mass of 22.6 kDa. It migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for RAC2 recombinant protein

Background: Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.

Description: A DNA sequence encoding the human RAC2 (P15153) (Met 1-Cys 189) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4506381
• NCBI GeneID: 5880
• NCBI Accession #: NP_002863.1
• NCBI GenBank Nucleotide #: NM_002872.4
• UniProt Accession #: P15153; Q9UDJ4; AAB22207
• Molecular Weight: 21,429 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 2
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• NCBI Official Symbol: RAC2
• NCBI Official Synonym Symbols: Gx; EN-7; HSPC022; p21-Rac2
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 2
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 2
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC2

NCBI Description

This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]

Uniprot Description

RAC2: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Interacts with DOCK2, which may activate it. Hematopoietic specific. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: actin filament; cytosol; focal adhesion; lamellipodium; phagocytic vesicle membrane; plasma membrane

Molecular Function: GTP binding

Biological Process: actin filament organization; platelet activation; regulation of hydrogen peroxide metabolic process; regulation of small GTPase mediated signal transduction; signal transduction

Disease: Neutrophil Immunodeficiency Syndrome