Typical Testing Data/Standard Curve (for reference only)
IL-18 elisa kit
IL-18 (Rat) ELISA Kit
Synonyms
IL-18; IL-18 (Rat) ELISA Kit; IL-18 elisa kit
Sequence Length
193
Samples
Rat serum, plasma, body fluids, tissue lysates or cell culture supernates
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
<1 pg/ml
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The %CV of intra-assay is < 10%
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. The %CV of inter-assay is < 10%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-18 elisa kit
Intended Uses: The rat IL-18 ELISA has been designed for the quantitative detection of IL-18 concentrations in rat serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only,
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-18 polyclonal antibody is precoated onto the 96 well plate. The biotin conjugated anti-IL-18 polyclonal antibody is used as the detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently and washed with wash buffer. Avidin-Biotin-Peroxidase Conjugate is added, and unbound conjugate is washed away with wash buffer. TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiter plate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample.
Background/Introduction: Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in humans is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cellmediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-18 polyclonal antibody is precoated onto the 96 well plate. The biotin conjugated anti-IL-18 polyclonal antibody is used as the detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently and washed with wash buffer. Avidin-Biotin-Peroxidase Conjugate is added, and unbound conjugate is washed away with wash buffer. TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiter plate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample.
Background/Introduction: Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in humans is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cellmediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,347 Da
NCBI Official Full Name
interleukin-18
NCBI Official Symbol
IL18
NCBI Protein Information
interleukin-18
UniProt Protein Name
Interleukin-18
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL-18; IFN-gamma-inducing factor; IL-1 gamma
UniProt Entry Name
IL18_BOVIN
Uniprot Description
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Research Articles on IL-18
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Product Notes
The IL-18 il18 (Catalog #AAA495380) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL-18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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