anti-Human FA12 Antibody | anti-FA12 antibody

FA12 (heavy chain, Cleaved-Arg372) Antibody

Cat. Num. AAA855070

• Gene Names: F12; HAF; HAE3; HAEX
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: FA12; FA12 (heavy chain; Cleaved-Arg372) Antibody; Coagulation factor XII [Precursor]; EC 3.4.21.38; Hageman factor; HAF; Coagulation factor XIIa heavy chain; Beta-factor XIIa part 1; Beta-factor XIIa part 2; Coagulation factor XIIa light chain; F12; anti-FA12 antibody

• Reactivity: Human
• Specificity: FA12 (heavy chain, Cleaved-Arg372) antibody detects endogenous levels of fragment of activated FA12 resulting from cleavage adjacent to Arg372.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 615

• Applicable Applications for anti-FA12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human FA12.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 1hour), using FA12 (heavy chain, Cleaved-Arg372) antibody.)

References

Cool D.E., J. Biol. Chem. 262:13662-13673(1987). Tripodi M., Nucleic Acids Res. 14:3146-3146(1986). Cool D.E., J. Biol. Chem. 260:13666-13676(1985).

NCBI and Uniprot Product Information

• NCBI GI #: 145275213
• NCBI GeneID: 2161
• NCBI Accession #: NP_000496.2
• NCBI GenBank Nucleotide #: NM_000505.3
• UniProt Accession #: P00748; P78339
• Molecular Weight: 67,792 Da
• NCBI Official Full Name: coagulation factor XII preproprotein
• NCBI Official Synonym Full Names: coagulation factor XII
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: HAF; HAE3; HAEX
• NCBI Protein Information: coagulation factor XII
• UniProt Protein Name: Coagulation factor XII
• UniProt Gene Name: F12
• UniProt Synonym Gene Names: HAF

NCBI Description

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Uniprot Description

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.