Rabbit Glucagon Antibody | anti-GCG antibody

Glucagon

Cat. Num. AAA397223

• Gene Names: GCG; GLP1; GLP2; GRPP; GLP-1
• Reactivity: Human, Rat, Pig
• Applications: Immunohistochemistry
• Synonyms: Glucagon; anti-GCG antibody

• Host: Rabbit
• Reactivity: Human, Rat, Pig
• Form/Format: Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
• Sequence Length: 180

• Applicable Applications for anti-GCG antibody: Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Dilution: 1:50
• Immunogen: Porcine pancreatic Glucagon-BSA conjugate
• Positive Control: Pancreas
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: No protease pre-treatment required. Improvement may be achieved by HIER.
• Pre-Treatment: No protease pre-treatment required. Improvement may be achieved by HIER.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for anti-GCG antibody

The antibody is well suited for the detection of glucagon in tissue sections of brain, pancreas and stomach. Glucagon is a polypeptide which is produced by s.c. A-cells of the pancreatic islets. It is the counterpart of insulin in the regulation of blood sugar levels. Except of the pancreas, glucagon exists also in endocrine cells of the stomach and in neurons of the brain. Frequently it has been found in pancreatic tumours and in rectal carcinoids, which can be responsible for glucagonoma syndromes. Glucagon.

References

1. Böttcher G, Ahrén B, Lundquist I, Sundler F. (1989) Peptide YY: intrapancreatic localization and effects on insulin and glucagon secretion in the mouse. Pancreas. 4(3):282-8. 2. Riedel MJ, Asadi A, Wang R, Ao Z, Warnock GL, Kieffer TJ. (2012) Immunohistochemical characterisation of cells co-producing insulin and glucagon in the developing human pancreas. Diabetologia. 55(2):372-81. 3. Sebastian Barg, Juris Galvanovskis, Sven O. Göpel, Patrik Rorsman, and Lena Eliasson (2000) Tight Coupling Between Electrical Activity and Exocytosis in Mouse Glucagon-Secreting ?-Cells DIABETES, VOL. 49;1500-1510. 4. Anna Wendt, Bryndis Birnir, Karsten Buschard, et al. (2004) Glucose Inhibition of Glucagon Secretion From Rat ?-Cells Is Mediated by GABA Released From Neighboring beta-Cells. Diabetes Vol. 53; 1038-1045.

NCBI and Uniprot Product Information

• NCBI GI #: 4503945
• NCBI GeneID: 2641
• NCBI Accession #: NP_002045.1
• NCBI GenBank Nucleotide #: NM_002054.4
• UniProt Accession #: P01275; Q53TP6; A6NN65
• Molecular Weight: 20,909 Da
• NCBI Official Full Name: glucagon preproprotein
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: GCG
• NCBI Official Synonym Symbols: GLP1; GLP2; GRPP; GLP-1
• NCBI Protein Information: glucagon
• UniProt Protein Name: Glucagon
• Protein Family: Glucagon
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2

NCBI Description

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Uniprot Description

Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.