Dicer 1, Ribonuclease Type III Recombinant Protein | DICER1 recombinant protein

Recombinant Dicer 1, Ribonuclease Type III (DICER1)

Cat. Num. AAA2033165

• Gene Names: DICER1; DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Dicer 1; Ribonuclease Type III; Recombinant Dicer 1; Ribonuclease Type III (DICER1); Dicer; HERNA; Double-Stranded RNA-Specific Endoribonuclease; Helicase with RNase motif; Helicase MOI; DICER1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: LQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LSYQIRGDFS RNGKRTVFLV NSANQVAQQV SAVRTHSDLK VGEYSNLEVN ASWTKERWNQ EFTKHQVLIM TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCENCPSCP RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDLVVLDRYTSQP CEIVVDCGPF TDRSGLYERL LMELEEALNF INDCNISVHS
• Sequence Length: 1922

• Applicable Applications for DICER1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 35.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Leu6~Ser290
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 152012889
• NCBI GeneID: 23405
• Molecular Weight: 92,726 Da
• NCBI Official Full Name: Dicer 1, ribonuclease type III
• NCBI Official Synonym Full Names: dicer 1 ribonuclease III
• NCBI Official Symbol: DICER1
• NCBI Official Synonym Symbols: DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
• NCBI Protein Information: endoribonuclease Dicer
• UniProt Protein Name: Endoribonuclease Dicer
• Protein Family: Endoribonuclease
• UniProt Gene Name: DICER1
• UniProt Synonym Gene Names: DICER; HERNA; KIAA0928; Helicase MOI
• UniProt Entry Name: DICER_HUMAN

NCBI Description

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.

Protein type: Cell development/differentiation; Helicase; EC 3.1.26.3; Ribonuclease; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: axon; cytoplasm; cytosol; dendrite; ER-Golgi intermediate compartment; growth cone; nucleus

Molecular Function: ATP binding; deoxyribonuclease I activity; DNA binding; double-stranded RNA binding; endoribonuclease activity; helicase activity; metal ion binding; miRNA binding; protein binding; protein domain specific binding; ribonuclease III activity; siRNA binding

Biological Process: angiogenesis; branching morphogenesis of a tube; cardiac muscle cell development; cartilage development; cerebral cortex development; defense response to virus; DNA fragmentation during apoptosis; embryonic hindlimb morphogenesis; gene expression; hair follicle morphogenesis; inner ear receptor cell development; lung development; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, production of miRNAs; mRNA stabilization; multicellular organism growth; myelin formation in the peripheral nervous system; myoblast cell differentiation involved in skeletal muscle regeneration; negative regulation of transcription from RNA polymerase II promoter; nerve development; neurite morphogenesis; olfactory bulb interneuron differentiation; positive regulation of myelination; positive regulation of Schwann cell differentiation; post-embryonic development; pre-microRNA processing; regulation of cell cycle; regulation of neuron differentiation; regulation of oligodendrocyte differentiation; regulation of viral genome replication; reproductive structure development; RNA interference, conversion of ds siRNA to ss siRNA; RNA interference, production of siRNA; RNA interference, siRNA loading onto RISC; RNA interference, targeting of mRNA for destruction; RNA-mediated gene silencing; spinal cord motor neuron differentiation; spindle assembly; spleen development; stem cell maintenance; zygote asymmetric cell division

Disease: Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Pleuropulmonary Blastoma; Rhabdomyosarcoma, Embryonal, 2