Western Blot (WB)
(Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit ECRG4 Polyclonal Antibody | anti-C2orf40 antibody
ECRG4 Antibody
Gene Names
C2orf40; ECRG4
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
ECRG4 Antibody is affinity chromatography purified via peptide column.
Synonyms
ECRG4; Polyclonal Antibody; ECRG4 Antibody; ECRG4 Antibody: ECRG4; UNQ761/PRO1508; Augurin; Esophageal cancer-related gene 4 protein; anti-C2orf40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ECRG4 antibody is predicted to not cross-react with other ECRG family members.
Purity/Purification
ECRG4 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; Supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-C2orf40 antibody
ELISA, Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 ug/mL.
Antibody can also be used for immunocytochemistry starting at 5 ug/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples.
All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher.
Antibody can also be used for immunocytochemistry starting at 5 ug/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples.
All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (75%)
Immunogen
ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4. The immunogen is located within the last 50 amino acids of ECRG4.
Positive Control
1) Cat. No. MBS151603 - HeLa Cell Lysate
2) Cat. No. MBS154246 - HeLa Cell Slide
2) Cat. No. MBS154246 - HeLa Cell Slide
Conjugate
Unconjugated
Preparation and Storage
ECRG4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 μg/mL.)
Related Product Information for anti-C2orf40 antibody
ECRG4 Antibody: The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
augurin
NCBI Official Synonym Full Names
chromosome 2 open reading frame 40
NCBI Official Symbol
C2orf40
NCBI Official Synonym Symbols
ECRG4
NCBI Protein Information
augurin; esophageal cancer related gene 4 protein; esophageal cancer-related gene 4 protein
UniProt Protein Name
Augurin
UniProt Gene Name
C2orf40
UniProt Synonym Gene Names
ECRG4
UniProt Entry Name
AUGN_HUMAN
Uniprot Description
C2orf40: Probable hormone that may induce senescence of oligodendrocyte and neural precursor cells, characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1 and CCND3 proteasomal degradation. Belongs to the augurin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q12.2
Cellular Component: extracellular space; transport vesicle
Biological Process: cyclin catabolic process
Research Articles on C2orf40
Similar Products
Product Notes
The C2orf40 c2orf40 (Catalog #AAA151199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECRG4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ECRG4 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB), Immunocytochemistry (ICC). ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the C2orf40 c2orf40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECRG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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