Rabbit SOCS1 Antibody | anti-SOCS1 antibody

Rabbit anti SOCS-1 antibody

Cat. Num. AAA462131

• Gene Names: SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: SOCS1; Rabbit anti SOCS-1 antibody; Rabbit anti-SOCS-1; Suppressor of Cytokine Signaling 1 (SOCS1); SSI1; TIP3; CISH1; Cish1; SSI-1; JAK binding protein (JAB); STAT induced SH3 protein 1; cytokine-inducible SH2 protein (CIS1); anti-SOCS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Specificity: This antibody recognizes ~24 kDa of SOCS-1 protein from human and mouse origins. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer.
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-SOCS1 antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 0.5-2 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to C-terminus of SOCS-1 protein from human and mouse origins.
• Positive Control: Mouse liver Lysate
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-SOCS1 antibody

Cytokines induce a variety of biological responses by binding to specific cell surface receptors and activating cytoplasmic signal transduction pathways which are suppressed by other transcriptional factors. Proteins that mediate these signals from the cell surface to the nucleus include JAKs and STATs, while SOCS (Suppressor of cytokine signaling) proteins inhibit those signaling. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine inducible SH2 containing protein). Structurally, the SOCS proteins are composed of an N terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C terminal motif named the SOCS box. SOCS 1 inhibition of signal transduction appears to occur by binding to and inhibiting the catalytic activity of members of JAK family of cytoplasmic kinases (direct JAK2 kinase inhibition). SOCS1 is an important negative regulator of JAK-STAT signal pathway.

References

Hongwei Qin, et al. IFN-beta-induced SOCS-1 negatively regulates CD40 gene expression in macrophages and microglia. The FASEB Journal. 2006;20:985-987.

NCBI and Uniprot Product Information

• NCBI GI #: 4507233
• NCBI GeneID: 8651
• NCBI Accession #: NP_003736.1
• NCBI GenBank Nucleotide #: NM_003745.1
• UniProt Accession #: O15524; O15097; Q9NSA7; Q4JHT5
• Molecular Weight: 24 kDa
• NCBI Official Full Name: suppressor of cytokine signaling 1
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 1
• NCBI Official Symbol: SOCS1
• NCBI Official Synonym Symbols: JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
• NCBI Protein Information: suppressor of cytokine signaling 1; TIP-3; OTTHUMP00000160221; JAK binding protein; JAK-binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; cytokine-inducible SH2 protein 1
• UniProt Protein Name: Suppressor of cytokine signaling 1
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS1
• UniProt Synonym Gene Names: SSI1; TIP3
• UniProt Entry Name: SOCS1_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq]

Uniprot Description

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival

By similarity. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. Ref.7 Ref.8

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV

By similarity. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1

By similarity. Interacts (via SH2 domain and SOCS box) with TRIM8

By similarity. Interacts with CUL2. Ref.7

Tissue specificity: Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

Induction: By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families.

Domain: The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box

Sequence similarities: Contains 1 SH2 domain.Contains 1 SOCS box domain.