Rabbit RAP1A Antibody | anti-RAP1A antibody

RAP1A Antibody

Cat. Num. AAA8505546

• Gene Names: RAP1A; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: RAP1A; RAP1A Antibody; KREV-1; KREV1; RAP1; SMGP21; anti-RAP1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 184

• Applicable Applications for anti-RAP1A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human RAP1A
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of C6 cell and spleen cell lysate using RAP1A antibody.)

Related Product Information for anti-RAP1A antibody

Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1, 2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1, 2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).

Product Categories/Family for anti-RAP1A antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Bos, J. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 369-377. 2. Caron, E. (2003) J. Cell Sci. 116, 435-440. 3. Song, C. et al. (2002) Oncogene 21, 8105-8113. 4. Rong, R. et al. (2003) J. Biol. Chem. 278, 52497-52503. 5. Taira, K. et al. (2004) J. Biol. Chem. 279, 49488-49496. 6. Choi, S. and Han, J. (2005) EMBO J. 24, 985-996.

NCBI and Uniprot Product Information

• NCBI GI #: 58331202
• NCBI GeneID: 5906
• NCBI Accession #: NP_001010935.1
• NCBI GenBank Nucleotide #: NM_001010935.2
• UniProt Accession #: P62834; P10113
• Molecular Weight: 21kDa
• NCBI Official Full Name: ras-related protein Rap-1A
• NCBI Official Synonym Full Names: RAP1A, member of RAS oncogene family
• NCBI Official Symbol: RAP1A
• NCBI Official Synonym Symbols: RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• NCBI Protein Information: ras-related protein Rap-1A
• UniProt Protein Name: Ras-related protein Rap-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP1A
• UniProt Synonym Gene Names: KREV1

NCBI Description

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.