Rabbit RAP1A Polyclonal Antibody | anti-RAP1A antibody

RAP1A Polyclonal Antibody

Cat. Num. AAA126616

• Gene Names: RAP1A; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: RAP1A; Polyclonal Antibody; RAP1A Polyclonal Antibody; KREV-1; KREV1; RAP1; SMGP21; anti-RAP1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 184

• Applicable Applications for anti-RAP1A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human RAP1A
• Calculated Molecular Weight: 21kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RAP1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Related Product Information for anti-RAP1A antibody

Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).

Product Categories/Family for anti-RAP1A antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 51338607
• NCBI GeneID: 5906
• UniProt Accession #: P62834; P10113
• Molecular Weight: 184
• NCBI Official Full Name: Ras-related protein Rap-1A
• NCBI Official Synonym Full Names: RAP1A, member of RAS oncogene family
• NCBI Official Symbol: RAP1A
• NCBI Official Synonym Symbols: RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• NCBI Protein Information: ras-related protein Rap-1A; Ras-related protein Krev-1; GTP-binding protein smg p21A
• UniProt Protein Name: Ras-related protein Rap-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP1A
• UniProt Synonym Gene Names: KREV1
• UniProt Entry Name: RAP1A_HUMAN

NCBI Description

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3.

Protein type: Tumor suppressor; G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; cytoplasm; early endosome; plasma membrane; cell junction; cytosol

Molecular Function: GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity

Biological Process: platelet activation; microvillus biogenesis; protein transport; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction