Rabbit P2RY12 Antibody | anti-P2RY12 antibody

P2RY12 Antibody

Cat. Num. AAA9386601

• Gene Names: P2RY12; HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: nogen affinity purified
• Synonyms: P2RY12; P2RY12 Antibody; anti-P2RY12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Specificity: P2RY12 Antibody detects endogenous levels of total P2RY12
• Purity/Purification: nogen affinity purified
• Concentration: 1.0mg/ml (varies by lot)
• Sequence: MQAVDNLTSA PGNTSLCTRD YKITQVLFPL LYTVLFFVGL ITNGLAMRIF FQIRSKSNFI IFLKNTVISD LLMILTFPFK ILSDAKLGTG PLRTFVCQVT SVIFYFTMYI SISFLGLITI DRYQKTTRPF KTSNPKNLLG AKILSVVIWA FMFLLSLPNM ILTNRQPRDK NVKKCSFLKS EFGLVWHEIV NYICQVIFWI NFLIVIVCYT LITKELYRSY VRTRGVGKVP RKKVNVKVFI IIAVFFICFV PFHFARIPYT LSQTRDVFDC TAENTLFYVK ESTLWLTSLN ACLDPFIYFF LCKSFRNSLI SMLKCPNSAT SLSQDNRKKE QDGGDPNEET PM
• Sequence Length: 342

• Applicable Applications for anti-P2RY12 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB 1:500-1:2000; IHC 1:50-1:200; Optimal dilutions/concentrations should be determined by the end user.
• Immunogen: A synthesized peptide derived from human P2RY12
• Function: Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation.
• Subcellular Location: Mitochondrion;Plasma Membrane
• Tissue Specificity: Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.
• Similarity: The transmembrane domain is composed of seven transmembrane helices; most of these are not strictly perpendicular to the plane of the membrane, but are tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Antagonists such as AZD1283 can bind to an overlapping site, but block the inward movement of the transmembrane helices (PubMed:24670650, PubMed:24784220).Belongs to the G-protein coupled receptor 1 family.
• Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 °C.Stable for 12 months from date of receipt.

Western blot

(Western blot analysis P2RY12 using Jurkat whole cell lysates)

Immunohistochemistry

(P2RY12 Antibody at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

NCBI and Uniprot Product Information

• NCBI GI #: 12232483
• NCBI GeneID: 64805
• NCBI Accession #: NP_073625.1
• NCBI GenBank Nucleotide #: NM_022788.4
• UniProt Accession #: Q9H244; Q546J7; D3DNJ5
• Molecular Weight: 39 kDa
• NCBI Official Full Name: P2Y purinoceptor 12
• NCBI Official Synonym Full Names: purinergic receptor P2Y12
• NCBI Official Symbol: P2RY12
• NCBI Official Synonym Symbols: HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
• NCBI Protein Information: P2Y purinoceptor 12
• UniProt Protein Name: P2Y purinoceptor 12
• Protein Family: P2Y purinoceptor
• UniProt Gene Name: P2RY12
• UniProt Synonym Gene Names: HORK3; P2Y12; ADPG-R; P2Y(ADP)

NCBI Description

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

P2Y12: Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. Defects in P2RY12 are the cause of bleeding disorder platelet-type 8 (BDPLT8). A condition characterized by mild to moderate mucocutaneous bleeding, and excessive bleeding after surgery or trauma. The defect is due to severe impairment of platelet response to ADP resulting in defective platelet aggregation. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: cell surface; integral to plasma membrane; intrinsic to membrane; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; platelet ADP receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; substrate-bound cell migration, cell extension

Disease: Bleeding Disorder, Platelet-type, 8