Rabbit GOT1 Polyclonal Antibody | anti-GOT1 antibody

GOT1 Antibody (C-term)

Cat. Num. AAA9203497

• Gene Names: GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GOT1; Polyclonal Antibody; GOT1 Antibody (C-term); Aspartate aminotransferase; cytoplasmic; cAspAT; Cysteine aminotransferase; Cysteine transaminase; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A; anti-GOT1 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-381 amino acids from the C-terminal region of human GOT1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 352-381
• Sequence Length: 392

• Applicable Applications for anti-GOT1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of GOT1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene.)

Immunohistochemistry (IHC)

(GOT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GOT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GOT1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GOT1 antibody

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Product Categories/Family for anti-GOT1 antibody

Cancer; Metabolism; Signal Transduction

References

Franke, A., et al. Nat. Genet. 42(4):292-294(2010)
Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
Campos, J., et al. Eur. J. Intern. Med. 20 (3), E53-E56 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4504067
• NCBI GeneID: 2805
• NCBI Accession #: NP_002070.1
• NCBI GenBank Nucleotide #: NM_002079.2
• UniProt Accession #: P17174; Q5VW80; B2R6R7; B7Z7E9
• Molecular Weight: 46248
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1, soluble
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: AST1; cCAT; GIG18; cAspAT; ASTQTL1
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Protein transport protein
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.3; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10q24.1-q25.1

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; aspartate biosynthetic process; glucose metabolic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; cellular response to insulin stimulus; methionine salvage; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate metabolic process; aspartate catabolic process; glutamate catabolic process to 2-oxoglutarate; amino acid biosynthetic process; polyamine metabolic process

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1