Rabbit anti-Human PRMT5 Polyclonal Antibody | anti-PRMT5 antibody

PRMT5, CT (C487) (Protein Methyltransferase JBP1, Protein Arginine N-methyltransferase 5, Histone-arginine N-methyltransferase PRMT5, Shk1 Kinase-binding Protein 1 Homolog, SKB1 Homolog, SKB1Hs, Jak-binding Protein 1, 72kD ICln-Binding Protein, HRMT1L5, I

Cat. Num. AAA6494425

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PRMT5; Polyclonal Antibody; CT (C487) (Protein Methyltransferase JBP1; Protein Arginine N-methyltransferase 5; Histone-arginine N-methyltransferase PRMT5; Shk1 Kinase-binding Protein 1 Homolog; SKB1 Homolog; SKB1Hs; Jak-binding Protein 1; 72kD ICln-Binding Protein; HRMT1L5; I; anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PRMT5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRMT5 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PRMT5 Antibody (C-term C487) in HL60 cell line lysates (35ug/lane).PRMT5(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PRMT5 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003). 2. Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001). 3. Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999). 4. Gilbreth, M., et al., Proc. Natl. Acad. Sci. USA 95(25):14781-14786 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 20070220
• NCBI GeneID: 10419
• NCBI Accession #: NP_006100
• NCBI GenBank Nucleotide #: NM_006109
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7
• Molecular Weight: 66,311 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 5 isoform a
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog; histone-arginine N-methyltransferase PRMT5; jak-binding protein 1; shk1 kinase-binding protein 1 homolog
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity

Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation