Rabbit KRAS Antibody | anti-KRAS antibody
KRAS Antibody
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
KRAS; KRAS Antibody; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS3; RASK2; anti-KRAS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
188
Applicable Applications for anti-KRAS antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:200-1:500
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Recombinant protein of human KRAS
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Related Product Information for anti-KRAS antibody
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5, 6).
Product Categories/Family for anti-KRAS antibody
References
1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654. 2. Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. 3. Buday, L. and Downward, J. (1993) Cell 73, 611-620. 4. Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431. 5. Bos, J.L. (1989) Cancer Res. 49, 4682-4689. 6. Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21kDa
NCBI Official Full Name
GTPase KRas isoform b
NCBI Official Synonym Full Names
KRAS proto-oncogene, GTPase
NCBI Official Symbol
KRAS
NCBI Official Synonym Symbols
NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
NCBI Protein Information
GTPase KRas
UniProt Protein Name
GTPase KRas
Protein Family
UniProt Gene Name
KRAS
UniProt Synonym Gene Names
KRAS2; RASK2
NCBI Description
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Uniprot Description
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
Research Articles on KRAS
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Product Notes
The KRAS kras (Catalog #AAA8504631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRAS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KRAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:200-1:500 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the KRAS kras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRAS, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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