Rabbit ITGA4 Antibody | anti-ITGA4 antibody

ITGA4 Antibody

Cat. Num. AAA8505926

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ITGA4; ITGA4 Antibody; CD49D; IA4; MGC90518; anti-ITGA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 1032

• Applicable Applications for anti-ITGA4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant protein of human ITGA4 WB 1:500-1:2000 IHC 1:50-1:200

Western Blot (WB)

(Western blot analysis of Jurkat cell lysate using ITGA4 antibody.)

Related Product Information for anti-ITGA4 antibody

Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1, 2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1, 2).A pair of important alpha4 integrins, alpha4beta1 and alpha4beta7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that alpha4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin alpha4 blocks binding to paxillin and promotes leading edge migration (7, 8).On SDS-PAGE, integrin alpha4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin alpha4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).

Product Categories/Family for anti-ITGA4 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100. 2. Liu, S. et al. (2000) J Cell Sci 113 (Pt 20), 3563-71. 3. Plow, E.F. et al. (2000) J Biol Chem 275, 21785-8. 4. Bonder, C.S. et al. (2005) Immunity 23, 153-63. 5. Arroyo, A.G. et al. (1999) Immunity 11, 555-66. 6. Yang, J.T. et al. (1995) Development 121, 549-60. 7. Nishiya, N. et al. (2005) Nat Cell Biol 7, 343-52. 8. Alon, R. et al. (2005) J Cell Biol 171, 1073-84. 9. Teixidó, J. et al. (1992) J Biol Chem 267, 1786-91. 10. Pujades, C. et al. (1996) Biochem J 313 (Pt 3), 899-908.

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.5
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4
• Molecular Weight: 115kDa
• NCBI Official Full Name: integrin alpha-4 isoform 1 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 4
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4
• UniProt Protein Name: Integrin alpha-4
• Protein Family: Integrin
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D

NCBI Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).