ITGA4 Antibody | anti-ITGA4 antibody

ITGA4 Antibody

Cat. Num. AAA8516052

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: ITGA4; ITGA4 Antibody; CD49D; IA4; MGC90518; anti-ITGA4 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: ITGA4 Antibody detects endogenous levels of total ITGA4
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1032

• Applicable Applications for anti-ITGA4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human ITGA4
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of Jurkat cell lysateusing ITGA4 antibody.)

Related Product Information for anti-ITGA4 antibody

Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1, 2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlaping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1, 2).Several alphaV subfamily members, including alphaVbeta3, alphaVbeta5, alphaVbeta1, are highly expressed in active endothelial cells and cancer cells (3-6) where they play a critical role in angiogenesis and tumor metastasis (7-9). Therefore, interest has focused on alphaV integrin as a key therapeutic target in the treatment of cancer (10-12).

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.5
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4
• Molecular Weight: 115kDa
• NCBI Official Full Name: integrin alpha-4 isoform 1 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 4
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4
• UniProt Protein Name: Integrin alpha-4
• Protein Family: Integrin
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D

NCBI Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).