Rabbit EEF2 Antibody | anti-EEF2 antibody

EEF2 Antibody

Cat. Num. AAA8505432

• Gene Names: EEF2; EF2; EF-2; EEF-2; SCA26
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: EEF2; EEF2 Antibody; EEF-2; EF2; anti-EEF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 858

• Applicable Applications for anti-EEF2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human EEF2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of HeLa cellJurkat cell line, using EEF2 antibody.)

Related Product Information for anti-EEF2 antibody

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5, 6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress-or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (10).

Product Categories/Family for anti-EEF2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Nairn, A.C. and Palfrey, H.C. (1987) J. Biol. Chem. 262, 17299-17303. 2. Ryazanov, A.G. et al. (1988) Nature 334, 170-173. 3. Carlberg, U. et al. (1990) Eur. J. Biochem. 191, 639-645. 4. Redpath, N.T. et al. (1993) Eur. J. Biochem. 213, 689-699. 5. Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7939-7943. 6. Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792. 7. Redpath, N.T. and Proud, C.G. (1993) Biochem. J. 293, 31-34. 8. Diggle, T. et al. (1998) Biochem. J. 336, 525-529. 9. Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.

NCBI and Uniprot Product Information

• NCBI GI #: 4503483
• NCBI GeneID: 1938
• NCBI Accession #: NP_001952.1
• NCBI GenBank Nucleotide #: NM_001961.3
• UniProt Accession #: P13639; Q58J86; B2RMP5; D6W618
• Molecular Weight: 95kDa
• NCBI Official Full Name: elongation factor 2
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 2
• NCBI Official Symbol: EEF2
• NCBI Official Synonym Symbols: EF2; EF-2; EEF-2; SCA26
• NCBI Protein Information: elongation factor 2
• UniProt Protein Name: Elongation factor 2
• Protein Family: Elongation factor
• UniProt Gene Name: EEF2
• UniProt Synonym Gene Names: EF2; EF-2

NCBI Description

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.