Rabbit anti-Human, Mouse CASP12 Polyclonal Antibody | anti-CASP12 antibody

CASP12 (Caspase 12, CASP12P1) (HRP)

Cat. Num. AAA6409571

• Gene Names: CASP12; CASP-12; CASP12P1
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: CASP12; Polyclonal Antibody; CASP12 (Caspase 12; CASP12P1) (HRP); anti-CASP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes CASP12. Species Crossreactivity: human, mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CASP12 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:10-1:100; WB: 1:500-1:1000; IHC: 1:20-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human CASP12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of mouse lung tissue, using CASP12 antibody.)

Product Categories/Family for anti-CASP12 antibody

Antibodies; Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 395398427
• NCBI GeneID: 100506742
• UniProt Accession #: Q6UXS9; D6RBN7
• Molecular Weight: 341
• NCBI Official Full Name: Inactive caspase-12
• NCBI Official Synonym Full Names: caspase 12 (gene/pseudogene)
• NCBI Official Symbol: CASP12
• NCBI Official Synonym Symbols: CASP-12; CASP12P1
• NCBI Protein Information: inactive caspase-12; caspase 12 pseudogene 1
• UniProt Protein Name: Inactive caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: CASP12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_HUMAN

NCBI Description

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: cytoplasm

Molecular Function: cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; unfolded protein response; regulation of inflammatory response; proteolysis