Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using EEF2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human eEF2 Polyclonal Antibody | anti-EEF2 antibody
eEF2 Rabbit pab Antibody
Gene Names
EEF2; EF2; EF-2; EEF-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
eEF2; Polyclonal Antibody; eEF2 Rabbit pab Antibody; EEF2 ; eEF2 ; EEF2; EEF-2; EF2; anti-EEF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
858
Applicable Applications for anti-EEF2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western Blot (WB): 1/500-1000
Immunohistochemistry (IHC): 1/50-100
Immunofluorescence (IF): 1/20
Immunohistochemistry (IHC): 1/50-100
Immunofluorescence (IF): 1/20
Immunogen
A synthetic -peptide of human eEF2
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using EEF2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using EEF2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-EEF2 antibody
Rabbit polyclonal to eEF2
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (10).
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (10).
References
1. Nairn, A.C. and Palfrey, H.C. (1987) J. Biol. Chem. 262, 17299-17303. 2. Ryazanov, A.G. et al. (1988) Nature 334, 170-173. 3. Carlberg, U. et al. (1990) Eur. J. Biochem. 191, 639-645. 4. Redpath, N.T. et al. (1993) Eur. J. Biochem. 213, 689-699. 5. Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7939-7943. 6. Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792. 7. Redpath, N.T. and Proud, C.G. (1993) Biochem. J. 293, 31-34. 8. Diggle, T. et al. (1998) Biochem. J. 336, 525-529. 9. Hovland, R. et al. (1999) FEBS Lett. 444, 97-101. 10. Proud, C. (2000) Translational Control of Gene Expression. Cold Spring Harbor Laboratory Press, NY,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
858
NCBI Official Full Name
elongation factor 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 2
NCBI Official Symbol
EEF2
NCBI Official Synonym Symbols
EF2; EF-2; EEF-2
NCBI Protein Information
elongation factor 2; polypeptidyl-tRNA translocase
UniProt Protein Name
Elongation factor 2
Protein Family
UniProt Gene Name
EEF2
UniProt Synonym Gene Names
EF2; EF-2
UniProt Entry Name
EF2_HUMAN
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Product Notes
The EEF2 eef2 (Catalog #AAA125227) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eEF2 Rabbit pab Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eEF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western Blot (WB): 1/500-1000 Immunohistochemistry (IHC): 1/50-100 Immunofluorescence (IF): 1/20. Researchers should empirically determine the suitability of the EEF2 eef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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