Rabbit BTRC Antibody | anti-BTRC antibody

BTRC Antibody

Cat. Num. AAA8501053

• Gene Names: BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: BTRC; BTRC Antibody; BETA-TRCP; FBW1A; FBXW1; FBXW1A; FWD1; MGC4643; bTrCP; bTrCP1; betaTrCP; anti-BTRC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 569

• Applicable Applications for anti-BTRC antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human BTRC
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of Kidneycell line, using BTRC antibody.)

Related Product Information for anti-BTRC antibody

beta-transducin repeat-containing protein (beta-TrCP or FBW1A) is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in cyclin F. F-box proteins act as substrate adaptors that target proteins containing a specific phosphorylated sequence element, referred to as a phosphodegron, to the SCF E3 ubiquitin ligase complex for ubiquitination (1, 2). beta-TrCP targets many important proteins with diverse functions, such as p53, H-Ras, Smad4, IkappaBalpha, beta-catenin, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation (3-5). Research studies have shown that inhibition of beta-TrCP expression has a demonstrated benefit in the treatment of prostate cancer (6).

Product Categories/Family for anti-BTRC antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Bour, S. et al. (2001) J Biol Chem 276, 15920-8. 2. Kusmierczyk, A.R. and Hochstrasser, M. (2008) Biol Chem 389, 1143-51. 3. Wan, M. et al. (2004) J Biol Chem 279, 14484-7. 4. Mailand, N. et al. (2006) Mol Cell 23, 307-18. 5. Frescas, D. and Pagano, M. (2008) Nat Rev Cancer 8, 438-49. 6. Gluschnaider, U. et al. (2010) PLoS One 5, e9060.

NCBI and Uniprot Product Information

• NCBI GI #: 4502477
• NCBI GeneID: 8945
• NCBI Accession #: NP_003930.1
• NCBI GenBank Nucleotide #: NM_003939.4
• UniProt Accession #: Q9Y297; Q5W141; Q5W142; Q9Y213; B5MD49
• Molecular Weight: 69kDa
• NCBI Official Full Name: F-box/WD repeat-containing protein 1A isoform 2
• NCBI Official Synonym Full Names: beta-transducin repeat containing E3 ubiquitin protein ligase
• NCBI Official Symbol: BTRC
• NCBI Official Synonym Symbols: FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
• NCBI Protein Information: F-box/WD repeat-containing protein 1A
• UniProt Protein Name: F-box/WD repeat-containing protein 1A
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: BTRC
• UniProt Synonym Gene Names: BTRCP; FBW1A; FBXW1A

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins (PubMed:10066435, PubMed:10497169, PubMed:10644755, PubMed:10835356, PubMed:11238952, PubMed:11359933, PubMed:11994270, PubMed:12791267, PubMed:12902344, PubMed:14603323, PubMed:14681206, PubMed:14988407, PubMed:15448698, PubMed:15917222, PubMed:16371461, PubMed:25503564, PubMed:25704143, PubMed:9859996). SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling (PubMed:12077367, PubMed:12820959). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2 (PubMed:10835356, PubMed:11238952, PubMed:14681206, PubMed:14603323). SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription (PubMed:10066435, PubMed:10497169, PubMed:10644755). Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22' (PubMed:10066435). SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed:25704143, PubMed:25503564). SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1 (). Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2 (PubMed:15917222). May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3 (PubMed:16371461).