Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using BTRC Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit BTRC Polyclonal Antibody | anti-BTRC antibody
BTRC Polyclonal Antibody
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using BTRC Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using BTRC Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U-2 OS cells using BTRC Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U-2 OS cells using BTRC Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]
Uniprot Description
BTRC: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Regulates the stability of CTNNB1 and participates in Wnt signaling. Interacts directly with SKP1 in the SCF complex. Interacts specifically with phosphorylated CTNNB1 and NFKBIA, ubiquitination substrates. Two alternatively spliced isoforms have been described.
Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q24.32
Cellular Component: nucleoplasm; SCF ubiquitin ligase complex; cytosol
Molecular Function: protein dimerization activity; protein phosphorylated amino acid binding; protein binding; ubiquitin-protein ligase activity; beta-catenin binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; viral reproduction; regulation of cell cycle; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; protein ubiquitination; positive regulation of circadian rhythm; protein amino acid dephosphorylation; signal transduction; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition of mitotic cell cycle; regulation of I-kappaB kinase/NF-kappaB cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; protein destabilization; negative regulation of transcription factor activity; mammary gland epithelial cell proliferation; regulation of circadian rhythm; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of smoothened signaling pathway; mitotic cell cycle; negative regulation of transcription, DNA-dependent
Research Articles on BTRC
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Product Notes
The BTRC btrc (Catalog #AAA2566155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTRC Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BTRC can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:50-1:200. Researchers should empirically determine the suitability of the BTRC btrc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTRC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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