Rabbit BTRC Polyclonal Antibody | anti-BTRC antibody

BTRC Antibody

Cat. Num. AAA9403922

• Gene Names: BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BTRC; Polyclonal Antibody; BTRC Antibody; BETA-TRCP; FBW1A; FBXW1; FBXW1A; FWD1; anti-BTRC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BTRC protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 569

• Applicable Applications for anti-BTRC antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human BTRC.
• Target Name: BTRC
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of Kidney cell line, using BTRC antibody.)

Related Product Information for anti-BTRC antibody

b-transducin repeat-containing protein (b-TrCP or FBW1A) is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in cyclin F. F-box proteins act as substrate adaptors that target proteins containing a specific phosphorylated sequence element, referred to as a phosphodegron, to the SCF E3 ubiquitin ligase complex for ubiquitination (1,2). b-TrCP targets many important proteins with diverse functions, such as p53, H-Ras, Smad4, IkBa, b-catenin, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation (3-5). Research studies have shown that inhibition of b-TrCP expression has a demonstrated benefit in the treatment of prostate cancer (6).

Product Categories/Family for anti-BTRC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502477
• NCBI GeneID: 8945
• NCBI Accession #: NP_003930.1
• NCBI GenBank Nucleotide #: NM_003939.4
• UniProt Accession #: Q9Y297; Q5W141; Q5W142; Q9Y213; B5MD49
• Molecular Weight: 65,049 Da
• NCBI Official Full Name: F-box/WD repeat-containing protein 1A isoform 2
• NCBI Official Synonym Full Names: beta-transducin repeat containing E3 ubiquitin protein ligase
• NCBI Official Symbol: BTRC
• NCBI Official Synonym Symbols: FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
• NCBI Protein Information: F-box/WD repeat-containing protein 1A
• UniProt Protein Name: F-box/WD repeat-containing protein 1A
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: BTRC
• UniProt Synonym Gene Names: BTRCP; FBW1A; FBXW1A
• UniProt Entry Name: FBW1A_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

BTRC: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Regulates the stability of CTNNB1 and participates in Wnt signaling. Interacts directly with SKP1 in the SCF complex. Interacts specifically with phosphorylated CTNNB1 and NFKBIA, ubiquitination substrates. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleoplasm; SCF ubiquitin ligase complex; cytosol

Molecular Function: protein dimerization activity; protein phosphorylated amino acid binding; protein binding; ubiquitin-protein ligase activity; beta-catenin binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; circadian rhythm; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; viral reproduction; positive regulation of proteolysis; regulation of cell cycle; positive regulation of transcription, DNA-dependent; protein ubiquitination; positive regulation of circadian rhythm; signal transduction; protein amino acid dephosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of I-kappaB kinase/NF-kappaB cascade; G2/M transition of mitotic cell cycle; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; protein destabilization; negative regulation of transcription factor activity; mammary gland epithelial cell proliferation; regulation of circadian rhythm; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of smoothened signaling pathway; mitotic cell cycle; negative regulation of transcription, DNA-dependent