Western Blot (WB)
(Western blot analysis of extracts ofMouse braintissue, using RhoB antibody.)
Rabbit anti-Human, Mouse RhoB Antibody | anti-RhoB antibody
RhoB Antibody
Gene Names
RHOB; ARH6; ARHB; RHOH6; MST081; MSTP081
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
RhoB; RhoB Antibody; ARH6; ARHB; MST081; MSTP081; RHOH6; anti-RhoB antibody
Host
Rabbit
Reactivity
Human, Mouse
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
196
Applicable Applications for anti-RhoB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:20-1:50
IHC: 1:50-1:100
IF: 1:20-1:50
Immunogen
Recombinant protein of humanRHOB
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts ofMouse braintissue, using RhoB antibody.)
Western Blot (WB)
(Western blot analysis of extracts ofMouse braintissue, using RhoB antibody.)
Related Product Information for anti-RhoB antibody
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2, 3). RhoB functions in the regulation of cell shape, migration and adhesion (4). RhoB activity has also been shown to play a role in protein trafficking (5, 6) and in CXCR2-mediated chemotaxis (6). Inhibition of RhoB activity downstream of PKC? influences the degree of invasion and migration by glioblastoma cells (7), and RhoB expression has a negative affect on tumor growth in ovarian cancer (8).
Product Categories/Family for anti-RhoB antibody
References
1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63. 2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12. 3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9. 4. Wheeler, A.P. and Ridley, A.J. (2007) Exp Cell Res 313, 3505-16. 5. Sandilands, E. et al. (2007) J Cell Sci 120, 2555-64. 6. Neel, N.F. et al. (2007) J Cell Sci 120, 1559-71. 7. Baldwin, R.M. et al. (2008) Oncogene 27, 3587-95. 8. Couderc, B. et al. (2008) Cancer Gene Ther 15, 456-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22kDa
NCBI Official Full Name
rho-related GTP-binding protein RhoB
NCBI Official Synonym Full Names
ras homolog family member B
NCBI Official Symbol
RHOB
NCBI Official Synonym Symbols
ARH6; ARHB; RHOH6; MST081; MSTP081
NCBI Protein Information
rho-related GTP-binding protein RhoB
UniProt Protein Name
Rho-related GTP-binding protein RhoB
Protein Family
UniProt Gene Name
RHOB
UniProt Synonym Gene Names
ARH6; ARHB; h6
Uniprot Description
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
Research Articles on RhoB
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Product Notes
The RhoB rhob (Catalog #AAA8500002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RhoB Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RhoB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:100 IF: 1:20-1:50. Researchers should empirically determine the suitability of the RhoB rhob for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RhoB, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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