Rabbit anti-Human, Mouse HEY2 Antibody | anti-HEY2 antibody

Rabbit HEY2 Antibody

Cat. Num. AAA1624

• Gene Names: HEY2; GRL; CHF1; HRT2; HERP1; HESR2; bHLHb32; GRIDLOCK
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: HEY2; Rabbit HEY2 Antibody; Hairy/enhancer-of-split related with YRPW motif protein 2; Cardiovascular helix-loop-helix factor 1; hCHF1; Hairy and enhancer of split-related protein 2; HESR-2; Hairy-related transcription factor 2; hHRT2; HES-related repressor protein 2; Protein gridlock homolog; CHF1; GRL; HERP; HERP1; HRT2; anti-HEY2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Specificity: HEY2 antibody detects endogenous levels of total HEY2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 337

• Applicable Applications for anti-HEY2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500~1:3000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human HEY2.
• Preparation and Storage: Store at -20 degree C/1 year

Western blot

(Western blot analysis of extracts from K562 cells, using HEY2 antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 6912414
• NCBI GeneID: 23493
• NCBI Accession #: NP_036391.1
• NCBI GenBank Nucleotide #: NM_012259.2
• UniProt Accession #: Q9UBP5
• NCBI Official Full Name: hairy/enhancer-of-split related with YRPW motif protein 2
• NCBI Official Synonym Full Names: hairy/enhancer-of-split related with YRPW motif 2
• NCBI Official Symbol: HEY2
• NCBI Official Synonym Symbols: GRL; CHF1; HRT2; HERP1; HESR2; bHLHb32; GRIDLOCK
• NCBI Protein Information: hairy/enhancer-of-split related with YRPW motif protein 2; HRT-2; hCHF1; hHRT2; HESR-2; protein gridlock homolog; HES-related repressor protein 1; HES-related repressor protein 2; hairy-related transcription factor 2; cardiovascular helix-loop-helix factor 1; class B basic helix-loop-helix protein 32; hairy and enhancer of split-related protein 2; cardiovascular basic helix-loop-helix factor 1
• UniProt Protein Name: Hairy/enhancer-of-split related with YRPW motif protein 2
• Protein Family: Hairy/enhancer-of-split related with YRPW motif protein
• UniProt Gene Name: HEY2
• UniProt Synonym Gene Names: BHLHB32; CHF1; GRL; HERP; HERP1; HRT2; hCHF1; bHLHb32; HESR-2; HRT-2; hHRT2
• UniProt Entry Name: HEY2_HUMAN

NCBI Description

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HEY2: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'- CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Belongs to the HEY family.

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; Sin3 complex; transcriptional repressor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; sequence-specific DNA binding; histone deacetylase binding; transcription factor activity; transcription factor binding

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; Notch signaling pathway; cell fate commitment; ventricular cardiac muscle cell development; positive regulation of heart rate; negative regulation of transcription from RNA polymerase II promoter; protein-DNA complex assembly; regulation of auditory receptor cell differentiation; negative regulation of Notch signaling pathway; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; vasculogenesis; mesenchymal cell development; anterior/posterior axis specification; positive regulation of cardiac muscle cell proliferation