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SDS-PAGE

Zika Virus Envelope Recombinant Protein

Zika Virus Envelope Recombinant

Applications
ELISA, Functional Assay, Lateral Flow
Purity
> 80% Pure (SDS-PAGE). Affinity Chromatography
Synonyms
Zika Virus Envelope; Zika Virus Envelope Recombinant; Zika Virus Envelope recombinant protein
Ordering
For Research Use Only!
Host
Insect Cells
Purity/Purification
> 80% Pure (SDS-PAGE). Affinity Chromatography
Form/Format
Purified, Liquid; Phosphate Buffered Saline, pH 7.4
Concentration
3.7 mg/ml (Bradford Method) (varies by lot)
Applicable Applications for Zika Virus Envelope recombinant protein
ELISA (EIA), CLIA and Lateral Flow Assays (LFA)
Application Notes
To overcome flavivirus cross-reactivity in recombinant antigens to envelope and NS1 antigens are commonly used. IgG and IgM antibodies typically show a high sensitivity and specificity to these epitopes especially NS1 which is thought to contain more species -specific epitopes than the envelope proteins. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Preservative
0.09% Sodium Azide
Safety Notes(s)
Refer to the aproppiate Safety Data Sheet for additional information.
Inactivation
Not Applicable
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Thawing
Thaw at room temperature or 4°C. Cryo-concentration may occur on thaing which should not affect performance characteristics. If cryo-concentration is seen after the material is completely thawed, mix by inverting the container GENTLY several times before use.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships with Dry Ice.
Store at -20°C, avoid repeated freeze/thaw cycles.

SDS-PAGE

SDS-PAGE
Related Product Information for Zika Virus Envelope recombinant protein
Zika Virus Envelope Recomb.
Zika Virus Envelope Recombinant
Product contains Histidine tag.Reacts with both IgG and IgM.
The E protein composes the majority of the virion surface and it is
involved with replication, such as host cell binding and membrane fusion.
Covers the ectodomain of the envelope proteins.
M.W. on 12.5% SDS-PAGE is ~50Kd.
The sequence of the envelope protein is derived from the African strain (all strains share very high identity in this region).
The amino acid sequence is proprietary.
Product Categories/Family for Zika Virus Envelope recombinant protein

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Product Notes

The Zika Virus Envelope (Catalog #AAA319787) is a Recombinant Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Zika Virus Envelope can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), CLIA and Lateral Flow Assays (LFA). To overcome flavivirus cross-reactivity in recombinant antigens to envelope and NS1 antigens are commonly used. IgG and IgM antibodies typically show a high sensitivity and specificity to these epitopes especially NS1 which is thought to contain more species -specific epitopes than the envelope proteins. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Researchers should empirically determine the suitability of the Zika Virus Envelope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zika Virus Envelope, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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