Rabbit SPAK Polyclonal Antibody | anti-SPAK antibody

SPAK polyclonal antibody

Cat. Num. AAA3014914

• Gene Names: STK39; DCHT; PASK; SPAK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: SPAK; Polyclonal Antibody; SPAK polyclonal antibody; STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39; STK39; anti-SPAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SPAK polyclonal antibody detects endogenous levels of SPAK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SPAK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human SPAK
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of SPAK polyclonal antibody at 1:500 dilutionLaneA: CT26 whole cell lysateLaneB: PC12 whole cell lysateLaneC: A549 whole cell lysateLaneD: MCF-7 whole cell lysate)

Related Product Information for anti-SPAK antibody

SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitrostudies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response. Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3. WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380. Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315. Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity. SPAK is also phosphorylated at Ser311 by PKC? in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well.

NCBI and Uniprot Product Information

• NCBI GI #: 115430252
• NCBI GeneID: 27347
• NCBI Accession #: NP_037365.2
• NCBI GenBank Nucleotide #: NM_013233.2
• UniProt Accession #: Q9UEW8; O14774; Q53S90; Q53SL7; Q53SS1; Q9UER4
• Molecular Weight: 59,474 Da
• NCBI Official Full Name: STE20/SPS1-related proline-alanine-rich protein kinase
• NCBI Official Synonym Full Names: serine threonine kinase 39
• NCBI Official Symbol: STK39
• NCBI Official Synonym Symbols: DCHT; PASK; SPAK
• NCBI Protein Information: STE20/SPS1-related proline-alanine-rich protein kinase; STE20/SPS1 homolog; ste-20-related kinase; Ste20-like protein kinase; small intestine SPAK-like kinase; serine/threonine-protein kinase 39; proline-alanine-rich STE20-related kinase; serine threonine
• UniProt Protein Name: STE20/SPS1-related proline-alanine-rich protein kinase
• Protein Family: Sensor histidine kinase
• UniProt Gene Name: STK39
• UniProt Synonym Gene Names: SPAK; Ste-20-related kinase
• UniProt Entry Name: STK39_HUMAN

NCBI Description

This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008]

Uniprot Description

STLK3: May act as a mediator of stress-activated signals. The phosphorylated form forms a complex with WNK2. Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: Kinase, protein; Protein kinase, STE; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; FRAY subfamily

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: cytoskeleton; basolateral plasma membrane; cytoplasm; apical plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-serine phosphorylation; regulation of inflammatory response; positive regulation of potassium ion transport; peptidyl-threonine phosphorylation; response to stress; protein amino acid phosphorylation