Rabbit anti-Human Growth Hormone Releasing Hormone (GHRH) Antibody | anti-GHRH antibody

Polyclonal Antibody to Growth Hormone Releasing Hormone (GHRH)

Cat. Num. AAA2006543

• Gene Names: GHRH; GRF; INN; GHRF
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Growth Hormone Releasing Hormone (GHRH); Polyclonal Antibody to Growth Hormone Releasing Hormone (GHRH); anti-GHRH antibody

• Host: Rabbit
• Reactivity: Human
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GHRH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-3ug/mL; IHC: 5-30ug/mL; ICC: 5-30ug/mL; ; Optimal working dilutions must be determined by end user.
• Species: Homo sapiens (Human)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Human
• Immunogen: Recombinant GHRH (Cys19~Gly108 (Accession # P01286)) expressed in E.coli (MBS2011223)
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 4° for frequent use. Aliquot and store at -20°C for 24 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample:Recombinant GHRH, Human.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded brain tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Lung cancer Tissue))

Immunohistochemistry (IHC)

(Samples: Human Kidney Tissue; Primary Ab: 30ug/ml Rabbit Anti-Human GHRH AntibodySecond Ab: 2ug/mL HRP-Linked CaprineAnti-Rabbit IgG Polyclonal Antibody(Catalog: )

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Stomach Tissue;Primary Ab: 30ug/ml Rabbit Anti-Human GHRH AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: )

References

The expression of GHRH and its receptors in breast carcinomas with apocrine differentiation—further evidence of the presence of a GHRH pathway in these tumors. Bence K?vári MD, PhD, Semir Vranic MD, PhD, Caterina Marchio MD, PhD, Anna Sapino MD, PhD, Gábor Cserni MD, PhD (2017)

NCBI and Uniprot Product Information

• NCBI GI #: 296080717
• NCBI GeneID: 2691
• NCBI Accession #: NP_001171660.1
• NCBI GenBank Nucleotide #: NM_001184731.2
• UniProt Accession #: P01286; Q4KN10; Q5JYR1
• NCBI Official Full Name: somatoliberin isoform 2 preproprotein
• NCBI Official Synonym Full Names: growth hormone releasing hormone
• NCBI Official Symbol: GHRH
• NCBI Official Synonym Symbols: GRF; INN; GHRF
• NCBI Protein Information: somatoliberin
• UniProt Protein Name: Somatoliberin
• Protein Family: Somatoliberin
• UniProt Gene Name: GHRH
• UniProt Synonym Gene Names: GHRF; GRF; GHRH

NCBI Description

This gene encodes a member of the glucagon family of proteins. The encoded preproprotein is produced in the hypothalamus and cleaved to generate the mature factor, known as somatoliberin, which acts to stimulate growth hormone release from the pituitary gland. Variant receptors for somatoliberin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

GHRH: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: extracellular region; extracellular space; intracellular; terminal bouton

Molecular Function: growth hormone-releasing hormone activity; growth hormone-releasing hormone receptor binding

Biological Process: adenohypophysis development; adenylate cyclase-activating G-protein coupled receptor signaling pathway; cAMP-mediated signaling; cell-cell signaling; growth hormone secretion; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; positive regulation of circadian sleep/wake cycle, REM sleep; positive regulation of growth hormone secretion; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of multicellular organism growth; response to food