Rabbit Urocortin 3 (UCN3) Polyclonal Antibody | anti-UCN3 antibody

Polyclonal Antibody to Urocortin 3 (UCN3)

Cat. Num. AAA2007025

• Gene Names: Ucn3; ucn III
• Reactivity: Mus musculus (Mouse)
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Urocortin 3 (UCN3); Polyclonal Antibody; Polyclonal Antibody to Urocortin 3 (UCN3); anti-UCN3 antibody

• Host: Rabbit
• Reactivity: Mus musculus (Mouse)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-UCN3 antibody: Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
• Application Notes: WB: 0.5-3ug/mL; IHC: 5-30ug/mL; ICC: 5-30ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Rat
• Immunogen: Recombinant UCN3 (His24~Lys149 (Accession # Q924A4)) expressed in E.coli (MBS2010391).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4°C for frequent use.; Aliquot and store at -20°C for 24 months.; ; Stability Test:; Thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot:Sample: Recombinant UCN3, Mouse.)

Western Blot (WB)

(Western Blot:Sample: Mouse Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Liver Tissue; Primary Ab: 30ug/ml Rabbit Anti-Mouse UCN3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Colon Tissue;Primary Ab: 30ug/ml Rabbit Anti-Mouse UCN3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Lung Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Spleen Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 21492632
• NCBI GeneID: 83428
• NCBI Accession #: NP_112540.2
• NCBI GenBank Nucleotide #: NM_031250.5
• UniProt Accession #: Q924A4
• NCBI Official Full Name: urocortin-3 preproprotein
• NCBI Official Synonym Full Names: urocortin 3
• NCBI Official Symbol: Ucn3
• NCBI Official Synonym Symbols: ucn III
• NCBI Protein Information: urocortin-3
• UniProt Protein Name: Urocortin-3
• Protein Family: Urocortin
• UniProt Gene Name: Ucn3
• UniProt Synonym Gene Names: Ucn III

NCBI Description

This gene encodes a member of the corticotropin-releasing hormone peptide family that participates in coordinating autonomic, endocrine, and behavioral responses to stress. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional hormone. The pancreatic islets isolated from mice lacking the encoded protein secrete less insulin in response to high glucose concentrations. Transgenic mice overexpressing the encoded protein exhibit a metabolically favorable phenotype resisting obesity and hyperglycemia on a high-fat diet. [provided by RefSeq, Sep 2016]

Uniprot Description

UCN3: Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13|13 A1

Cellular Component: axon; axon terminus; extracellular region; extracellular space; neuron projection

Molecular Function: corticotropin-releasing hormone receptor 2 binding; G-protein coupled receptor binding; hormone activity; neuropeptide hormone activity; protein binding

Biological Process: adenylate cyclase-activating G-protein coupled receptor signaling pathway; cellular response to nutrient levels; digestion; neuropeptide signaling pathway; positive regulation of insulin secretion; positive regulation of membrane potential; response to stress