Western Blot (WB)
(Western blot analysis of extracts of Raji cellline, using BIK antibody.)
Rabbit anti-Human BIK Antibody | anti-BIK antibody
BIK Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
BIK; BIK Antibody; BP4; NBK; BIP1; anti-BIK antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
160
Applicable Applications for anti-BIK antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human BIK
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of Raji cellline, using BIK antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Raji cellline, using BIK antibody.)
Related Product Information for anti-BIK antibody
Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2 (1, 2). Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins (3). Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity (4).
Product Categories/Family for anti-BIK antibody
References
1. Boyd, J. M. et al. (1995) Oncogene 11, 1921-1928. 2. Han, J. et al. (1996) Mol. Cell. Biol. 16, 5857-5864. 3. Tong, Y. et al. (2001) Mol. Cancer Ther. 1, 95-102. 4. Verma, S. et al. (2001) J. Biol. Chem. 276, 4671-4676.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18kDa
NCBI Official Full Name
bcl-2-interacting killer
NCBI Official Synonym Full Names
BCL2 interacting killer
NCBI Official Symbol
BIK
NCBI Official Synonym Symbols
BP4; NBK; BIP1
NCBI Protein Information
bcl-2-interacting killer
UniProt Protein Name
Bcl-2-interacting killer
Protein Family
UniProt Gene Name
BIK
UniProt Synonym Gene Names
NBK
NCBI Description
The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011]
Uniprot Description
Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Research Articles on BIK
Similar Products
Product Notes
The BIK bik (Catalog #AAA8500086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BIK Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BIK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the BIK bik for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIK, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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