Rabbit anti-Mouse BIK Polyclonal Antibody | anti-BIK antibody

RABBIT ANTI BIK (N-TERMINAL)

Cat. Num. AAA221461

• Gene Names: BIK; BP4; NBK; BIP1
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: BIK; Polyclonal Antibody; RABBIT ANTI BIK (N-TERMINAL); anti-BIK antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk). Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis. ; ; Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-BIK antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 18kDa in Jurkat cell lysates.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: 15 amino acid peptide from near the amino terminus of human Bik.; Buffer Solution
• Antiserum Preparation: Antisera to Bik were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from Jurkat, human T-cell leukemia cells probed with Rabbit anti Human BIK (MBS221461) at 1(A) and 2(B) ug/ml)

Testing Data #2

(Imunocytochemical staining of Jurkat cells with Rabbit anti Human BIK (MBS221461))

Related Product Information for anti-BIK antibody

MBS221461 detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk). Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis. Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4502411
• NCBI GeneID: 638
• NCBI Accession #: NP_001188.1
• NCBI GenBank Nucleotide #: NM_001197.4
• UniProt Accession #: Q13323; Q16582; Q6FH93
• Molecular Weight: 18,016 Da
• NCBI Official Full Name: bcl-2-interacting killer
• NCBI Official Synonym Full Names: BCL2-interacting killer (apoptosis-inducing)
• NCBI Official Symbol: BIK
• NCBI Official Synonym Symbols: BP4; NBK; BIP1
• NCBI Protein Information: bcl-2-interacting killer; apoptosis inducer NBK; apoptosis-inducing NBK
• UniProt Protein Name: Bcl-2-interacting killer
• Protein Family: Bcl-2-interacting killer
• UniProt Gene Name: BIK
• UniProt Synonym Gene Names: NBK
• UniProt Entry Name: BIK_HUMAN

NCBI Description

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011]

Uniprot Description

BIK: accelerates programmed cell death. Binding to the apoptosis repressors Bcl-xL, Bcl-2, or BHRF1 (the Epstein-Barr virus homolog of Bcl-2) suppresses its death-promoting activity. Contains 1 Bcl-2 homology 3 (BH3) domain. Does not interact with Bax.

Protein type: Mitochondrial; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrial membrane; integral to membrane; endomembrane system

Molecular Function: BH domain binding; protein binding; protein heterodimerization activity

Biological Process: apoptotic mitochondrial changes; apoptosis; positive regulation of protein homooligomerization; male gonad development; spermatogenesis