Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using BIK (Phospho-Thr33) antibody (#A0053).)
anti-Human BIK Antibody | anti-BIK antibody
BIK (Phospho-Thr33) Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
BIK; BIK (Phospho-Thr33) Antibody; Apoptosis inducer NBK; BIKLK; BIP1; BP4; Bcl-2 interacting killer; NBK; anti-BIK antibody
Reactivity
Human
Specificity
BIK (Phospho-Thr33) antibody detects endogenous levels of BIK only when phosphorylated at threonine 33.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
160
Applicable Applications for anti-BIK antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
IHC: 1:50~1:100
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human BIK around the phosphorylation site of threonine 33 (G-M-TP-D-S).
All Sites
Human: Thr33
Preparation and Storage
Store at-20 degree C for 1 year
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using BIK (Phospho-Thr33) antibody (#A0053).)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using BIK (Phospho-Thr33) antibody (#A0053).)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using BIK (Phospho-Thr33) antibody (#A0053, Line 1 and 2).)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using BIK (Phospho-Thr33) antibody (#A0053, Line 1 and 2).)
Product Categories/Family for anti-BIK antibody
References
Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671-4676. Yan M. Li, Cancer Res., Nov 2003; 63: 7630-7633. Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443-449. Feng Dong, Infect. Immun., Mar 2005; 73: 1861-1864.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,016 Da
NCBI Official Full Name
bcl-2-interacting killer
NCBI Official Synonym Full Names
BCL2 interacting killer
NCBI Official Symbol
BIK
NCBI Official Synonym Symbols
BP4; NBK; BIP1
NCBI Protein Information
bcl-2-interacting killer
UniProt Protein Name
Bcl-2-interacting killer
Protein Family
UniProt Gene Name
BIK
UniProt Synonym Gene Names
NBK
NCBI Description
The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011]
Uniprot Description
Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Research Articles on BIK
Similar Products
Product Notes
The BIK bik (Catalog #AAA852100) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The BIK (Phospho-Thr33) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BIK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the BIK bik for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIK, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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